Mouse TCF7/TCF1 Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB8224
Conjugate
Catalog #
Key Product Details
Species Reactivity
Mouse
Applications
CyTOF-ready, Intracellular Staining by Flow Cytometry, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Rat IgG2B Clone # 812145
Product Specifications
Immunogen
E. coli-derived recombinant mouse TCF7/TCF1
Gly61-Gly116
Accession # Q00417
Gly61-Gly116
Accession # Q00417
Specificity
Detects mouse TCF7/TCF1 in ELISA and Western Blot.
Clonality
Monoclonal
Host
Rat
Isotype
IgG2B
Scientific Data Images for Mouse TCF7/TCF1 Antibody
Detection of Mouse TCF7/TCF1 by Western Blot.
Western blot shows lysates of EL-4 mouse lymphoblast cell line and mouse thymus tissue. PVDF membrane was probed with 1 µg/mL of Rat Anti-Mouse TCF7/TCF1 Monoclonal Antibody (Catalog # MAB8224) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). Specific bands were detected for TCF7/TCF1 at approximately 30-50 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Detection of TCF7/TCF1 in EL‑4 Mouse Cell Line by Flow Cytometry.
EL-4 mouse lymphoblast cell line was stained with Rat Anti-Mouse TCF7/TCF1 Monoclonal Antibody (Catalog # MAB8224, filled histogram) or isotype control antibody (Catalog # MAB0061, open histogram), followed by Allophycocyanin-conjugated Anti-Rat IgG Secondary Antibody (Catalog # F0113). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with saponin.Applications for Mouse TCF7/TCF1 Antibody
Application
Recommended Usage
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
Intracellular Staining by Flow Cytometry
2.5 µg/106 cells
Sample: EL‑4 mouse lymphoblast cell line fixed with paraformaldehyde and permeabilized with saponin
Sample: EL‑4 mouse lymphoblast cell line fixed with paraformaldehyde and permeabilized with saponin
Western Blot
1 µg/mL
Sample: EL‑4 mouse lymphoblast cell line and mouse thymus tissue
Sample: EL‑4 mouse lymphoblast cell line and mouse thymus tissue
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Reviewed Applications
Read 1 review rated 3 using MAB8224 in the following applications:
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: TCF7/TCF1
Long Name
Transcription Factor 7
Alternate Names
TCF1
Gene Symbol
TCF7
UniProt
Additional TCF7/TCF1 Products
Product Documents for Mouse TCF7/TCF1 Antibody
Product Specific Notices for Mouse TCF7/TCF1 Antibody
For research use only
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