Mouse TREM-1 Antibody
R&D Systems, part of Bio-Techne | Catalog # AF1187
Key Product Details
Validated by
Biological Validation
Species Reactivity
Validated:
Mouse
Cited:
Mouse
Applications
Validated:
Agonist Activity, CyTOF-ready, ELISA Capture (Matched Antibody Pair), Flow Cytometry, Immunocytochemistry, Western Blot
Cited:
Bioassay, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Product Specifications
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse TREM-1
Ala21-Ser202
Accession # Q9JKE2
Ala21-Ser202
Accession # Q9JKE2
Specificity
Detects mouse TREM-1 in ELISAs and Western blots. In sandwich immunoassays, less than 0.2% cross‑reactivity with recombinant human (rh) TREM-1 is observed.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Scientific Data Images for Mouse TREM-1 Antibody
TREM‑1 in Mouse Splenocytes.
TREM-1 was detected in immersion fixed mouse splenocytes treated with LPS (left panel) or untreated (right panel) using Goat Anti-Mouse TREM-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1187) at 15 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.Applications for Mouse TREM-1 Antibody
Application
Recommended Usage
Agonist Activity
Measured by its ability to stimulate TNF-alpha secretion by P388D1 mouse lymphoma cells (ATCC # TIB-63). Bouchon, A. et al. (2001) Nature, 410:1103 and Bouchon, A. et al. (2000) J. Immunology, 164:4991.
The ED50 for this effect is typically 1‑3 μg/mL.
The ED50 for this effect is typically 1‑3 μg/mL.
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
Flow Cytometry
0.25 µg/106 cells
Sample: Mouse blood GR-1+ granulocytes
Sample: Mouse blood GR-1+ granulocytes
Immunocytochemistry
5-15 µg/mL
Sample: Immersion fixed mouse splenocytes treated with LPS
Sample: Immersion fixed mouse splenocytes treated with LPS
Western Blot
0.1 µg/mL
Sample: Recombinant Mouse TREM-1 Fc Chimera (Catalog # 1187-TR)
Sample: Recombinant Mouse TREM-1 Fc Chimera (Catalog # 1187-TR)
Mouse TREM-1 Sandwich Immunoassay
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: TREM-1
TREM-1 (Triggering Receptor Expressed on Myeloid cells) is a type I transmembrane protein having a single Ig-like domain. It associates with the adapter protein, DAP12, to deliver an activating signal. Several other TREM family members have been reported that are structurally similar but share less than 30% amino acid identity. TREM-1 is expressed on blood neutrophils and a subset of monocytes, and expression is up-regulated by bacterial LPS. Engagement of TREM-1 with a monoclonal antibody leads to expression of IL-8, MCP-1, and TNF-alpha suggesting that this receptor plays an important role in inflammatory responses. TREM-1 is expressed at high levels on neutrophils of patients with microbial sepsis and in mice with LPS-induced shock. Blockade of TREM-1 with a TREM-1/Fc fusion protein protected mice against LPS-induced shock.
References
- Bouchon, A. (2000) J. Immunol. 164:4991.
- Bouchon, A. (2001) Nature 410:1103.
- Nathan, C. and A. Ding (2001) Nature Med. 7:530.
Long Name
Triggering Receptor Expressed on Myeloid Cells 1
Alternate Names
CD354, TREM1
Gene Symbol
TREM1
UniProt
Additional TREM-1 Products
Product Documents for Mouse TREM-1 Antibody
Product Specific Notices for Mouse TREM-1 Antibody
For research use only
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