Recombinant Mouse TREM-1 Fc Chimera Protein, CF
R&D Systems, part of Bio-Techne | Catalog # 1187-TR
Key Product Details
Source
NS0
Accession #
Structure / Form
Disulfide-linked homodimer
Conjugate
Unconjugated
Applications
Bioactivity
Product Specifications
Source
Mouse myeloma cell line, NS0-derived mouse TREM-1 protein
| Mouse TREM-1 (Ala21-Ser202) Accession # Q9JKE2 |
SIEGRMD | Human IgG1 (Pro100-Lys330) |
| N-terminus | C-terminus |
Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
N-terminal Sequence Analysis
Ala21
Predicted Molecular Mass
47 kDa (monomer)
SDS-PAGE
60-73 kDa, reducing conditions
Activity
Measured by its ability to block anti-TREM-1-induced TNF-alpha secretion by P388D1 mouse lymphoma cells.
The ED50 for this effect is 2-8 ng/mL in the presence of 0.5-2 µg/mL of Goat Anti-Mouse TREM-1 (Catalog # AF1187).
The ED50 for this effect is 2-8 ng/mL in the presence of 0.5-2 µg/mL of Goat Anti-Mouse TREM-1 (Catalog # AF1187).
Formulation, Preparation and Storage
1187-TR
| Formulation | Lyophilized from a 0.2 μm filtered solution in PBS. |
| Reconstitution |
Reconstitute at 100 μg/mL in sterile PBS.
|
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Background: TREM-1
TREM-1 (Triggering Receptor Expressed on Myeloid cells) is a type I transmembrane protein having a single Ig-like domain. It associates with the adapter protein, DAP12, to deliver an activating signal. Several other TREM family members have been reported that are structurally similar but share less than 30% amino acid identity. TREM-1 is expressed on blood neutrophils and a subset of monocytes, and expression is up-regulated by bacterial LPS. Engagement of TREM-1 with a monoclonal antibody leads to expression of IL-8, MCP-1, and TNF-alpha suggesting that this receptor plays an important role in inflammatory responses. TREM-1 is expressed at high levels on neutrophils of patients with microbial sepsis and in mice with LPS-induced shock. Blockade of TREM-1 with a TREM-1/Fc fusion protein protected mice against LPS-induced shock.
References
- Bouchon, A. (2000) J. Immunol. 164:4991.
- Bouchon, A. (2001) Nature 410:1103.
- Nathan, C. and A. Ding (2001) Nature Med. 7:530.
Long Name
Triggering Receptor Expressed on Myeloid Cells 1
Alternate Names
CD354, TREM1
Gene Symbol
TREM1
UniProt
Additional TREM-1 Products
Product Documents for Recombinant Mouse TREM-1 Fc Chimera Protein, CF
Product Specific Notices for Recombinant Mouse TREM-1 Fc Chimera Protein, CF
For research use only
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