Mouse TREML1/TLT-1 Biotinylated Antibody

R&D Systems, part of Bio-Techne | Catalog # BAF2424

R&D Systems, part of Bio-Techne
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Key Product Details

Species Reactivity

Mouse

Applications

ELISA Detection (Matched Antibody Pair), Western Blot

Label

Biotin

Antibody Source

Polyclonal Goat IgG

View all TREML1/TLT-1 Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse TREML1/TLT‑1
Gly18-Cys178
Accession # NP_082039

Specificity

Detects mouse TREML1/TLT‑1 in ELISAs and Western blots. In sandwich ELISAs, less than 0.03% cross-reactivity with recombinant human (rh) TREML1/TLT-1 and rhTREML2/TLT-2 is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Applications for Mouse TREML1/TLT-1 Biotinylated Antibody

Application
Recommended Usage

Western Blot

0.1 µg/mL
Sample: Recombinant Mouse TREML1/TLT-1 (Catalog # 2424-TL)

Mouse TREML1/TLT-1 Sandwich Immunoassay

ELISA Detection (Matched Antibody Pair)
Recommended Concentration: 0.1-0.4 µg/mL
Use in combination with these reagents:
  • Capture Reagent: Mouse TREML1/TLT-1 Antibody (Catalog # MAB24241)
  • Standard: Recombinant Mouse TREML1/TLT-1 Protein, CF (Catalog # 2424-TL)
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Reconstitution Buffer Available:
Size / Price
Qty

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: TREML1/TLT-1

Triggering receptor expressed on myeloid cells-like protein 1 (TREML1)/TREM-like transcript-1 (TLT‑1) is a 40 kDa type I transmembrane receptor and member of the TREM family of proteins (1). Mouse TREML1/TLT-1 is synthesized as a 317 amino acid (aa) precursor that contains a 20 aa signal sequence, a 155 aa extracellular domain (ECD), a 21 aa transmembrane region, and a 121 aa cytoplasmic domain (SwissProt #: Q8K558). The ECD contains an Ig-like V-type domain (aa 21‑122), and the cytoplasmic region contains a proline-rich region (aa 245‑275) and an immunoreceptor tyrosine-based inhibition motif (ITIM) characterized by the residues S/I/V/LXYXXV/L (1). A splicing variant produces a second isoform that has a six aa substitution for aa 173 in isoform 1. Mature mouse TREML1/TLT-1 shares 65% aa sequence identity with mature human TREML1/TLT-1. TREML1/TLT-1 is expressed exclusively in megakaryocytes and platelets where it colocalizes with CD62P in alpha‑granules in resting platelets and on the cell surface of platelets activated by thrombin (1‑3). The receptor’s expression is up‑regulated dramatically upon platelet activation (2). Antibodies to single-chain Fv fragments specific for TLT-1 inhibited thrombin-mediated human platelet aggregation suggesting its role in the regulation of aggregation (5). In addition, soluble fragments including the ECD can be released into serum by proteolysis (3). The phosphorylated TREML1/TLT-1 is able to interact with both SHP-1 and SHP-2 through ITIM (1, 4). SHP-2 interaction enhances Fc epsilonRI-mediated calcium signaling in rat basophilic leukemia cells (1). It is hypothesized that TREML1/TLT-1 plays a role in the innate and adaptive immune responses.

References

  1. Barrow, A.D. et al. (2004) J. Immunol. 172:5838.
  2. Washington, A.V. et al. (2004) Blood 104:1042.
  3. Gattis, J.L. et al. (2006) J. Biol. Chem. 281:13396.
  4. Washington, A.V. et al. (2002) Blood 100:3822.
  5. Giomarelli B. et al. (2007) Thromb. Haemost. 97:955.

Long Name

Triggering Receptor Expressed on Myeloid Cells-like 1

Alternate Names

TLT-1, TLT1

Entrez Gene IDs

340205 (Human); 71326 (Mouse)

Gene Symbol

TREML1

UniProt

NP_082039

Additional TREML1/TLT-1 Products

Product Documents for Mouse TREML1/TLT-1 Biotinylated Antibody

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Product Specific Notices for Mouse TREML1/TLT-1 Biotinylated Antibody

For research use only

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