Mouse TREML1/TLT-1 Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB24241

R&D Systems, part of Bio-Techne
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MAB24241
MAB24241-SP
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Key Product Details

Species Reactivity

Mouse

Applications

ELISA Capture (Matched Antibody Pair), Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Rat IgG2B Clone # 268515

View all TREML1/TLT-1 Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse TREML1/TLT‑1
Gly18-Cys178
Accession # NP_082039

Specificity

Detects mouse TREML1/TLT‑1 in ELISAs and Western blots. In ELISAs and Western blots, no cross-reactivity with recombinant human (rh) TREML1 or rhTREML2 is observed.

Clonality

Monoclonal

Host

Rat

Isotype

IgG2B

Applications for Mouse TREML1/TLT-1 Antibody

Application
Recommended Usage

Western Blot

1 µg/mL
Sample: Recombinant Mouse TREML1/TLT‑1 (Catalog # 2424-TL) under non-reducing conditions only

Mouse TREML1/TLT-1 Sandwich Immunoassay

ELISA Capture (Matched Antibody Pair)
Recommended Concentration: 2-8 µg/mL
Use in combination with these reagents:
  • Detection Reagent: Mouse TREML1/TLT-1 Biotinylated Antibody (Catalog # BAF2424)
  • Standard: Recombinant Mouse TREML1/TLT-1 Protein, CF (Catalog # 2424-TL)
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Reconstitution Buffer Available:
Size / Price
Qty

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: TREML1/TLT-1

Triggering receptor expressed on myeloid cells-like protein 1 (TREML1)/TREM-like transcript-1 (TLT‑1) is a 40 kDa type I transmembrane receptor and member of the TREM family of proteins (1). Mouse TREML1/TLT-1 is synthesized as a 317 amino acid (aa) precursor that contains a 20 aa signal sequence, a 155 aa extracellular domain (ECD), a 21 aa transmembrane region, and a 121 aa cytoplasmic domain. The ECD contains an Ig-like V-type domain (aa 21‑122), and the cytoplasmic region contains a proline-rich region (aa 245‑275) and an immunoreceptor tyrosine-based inhibition motif (ITIM) characterized by the residues S/I/V/LXYXXV/L (1). A splicing variant produces a second isoform that has a six aa substitution for aa 173 in isoform 1. Mature mouse TREML1/TLT-1 shares 65% aa sequence identity with mature human TREML1/TLT-1. TREML1/TLT-1 is expressed exclusively in megakaryocytes and platelets where it colocalizes with CD62P in alpha‑granules in resting platelets and on the cell surface of platelets activated by thrombin (1‑3). The receptor’s expression is up‑regulated dramatically upon platelet activation (2). Antibodies to single‑chain Fv fragments specific for TLT-1 inhibited thrombin-mediated human platelet aggregation suggesting its role in the regulation of aggregation (5). In addition, soluble fragments including the ECD can be released into serum by proteolysis (3). The phosphorylated TREML1/TLT-1 is able to interact with both SHP-1 and SHP-2 through ITIM (1, 4). SHP-2 interaction enhances Fc epsilonRI-mediated calcium signaling in rat basophilic leukemia cells (1). It is hypothesized that TREML1/TLT-1 plays a role in the innate and adaptive immune responses.

References

  1. Barrow, A.D. et al. (2004) J. Immunol. 172:5838.
  2. Washington, A.V. et al. (2004) Blood 104:1042.
  3. Gattis, J.L. et al. (2006) J. Biol. Chem. 281:13396.
  4. Washington, A.V. et al. (2002) Blood 100:3822.
  5. Giomarelli B. et al. (2007) Thromb. Haemost. 97:955.

Long Name

Triggering Receptor Expressed on Myeloid Cells-like 1

Alternate Names

TLT-1, TLT1

Entrez Gene IDs

340205 (Human); 71326 (Mouse)

Gene Symbol

TREML1

UniProt

NP_082039

Additional TREML1/TLT-1 Products

Product Documents for Mouse TREML1/TLT-1 Antibody

Product Datasheets

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Mouse TREML1/TLT-1 Antibody

For research use only

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