Mouse XCL1/Lymphotactin Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB486
Key Product Details
Species Reactivity
Validated:
Mouse
Cited:
Mouse
Applications
Validated:
ELISA Capture (Matched Antibody Pair), Western Blot
Cited:
Flow Cytometry, Neutralization
Label
Unconjugated
Antibody Source
Monoclonal Rat IgG2A Clone # 80222
Product Specifications
Immunogen
E. coli-derived recombinant mouse XCL1/Lymphotactin
Val22-Gly114
Accession # AAA56752
Val22-Gly114
Accession # AAA56752
Specificity
Detects mouse XCL1/Lymphotactin in ELISAs and Western blots. In sandwich immunoassays, less than 1% cross-reactivity with recombinant human XCL1/Lymphotactin is observed and no cross-reactivity with recombinant viral (rv) CMV UL147 is observed. In Western blots, 75% cross‑reactivity with rvCMV UL147 is observed and no cross-reactivity with recombinant human CCL1-3, 5, 7, 8, 11, 13-25, 28, recombinant mouse CCL1-7, 9, 11, 12, 15, 19, 21, 22, 24, 25, 27 or 28 is observed.
Clonality
Monoclonal
Host
Rat
Isotype
IgG2A
Applications for Mouse XCL1/Lymphotactin Antibody
Application
Recommended Usage
Western Blot
1 µg/mL
Sample: Recombinant Mouse XCL1/Lymphotactin (Catalog # 486-LT)
Sample: Recombinant Mouse XCL1/Lymphotactin (Catalog # 486-LT)
Mouse XCL1/Lymphotactin Sandwich Immunoassay
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: XCL1/Lymphotactin
Mouse XCL1, also known as Lymphotactin, and its human homologue (also named human SCM-1 and ATAC) belong to the C or gamma subfamily of chemokines. The C chemokines lack two (the 1st and 3rd) of the four invariant cysteine residues normally found in the CC and CXC chemokines and have an extended carboxy terminus. Mouse XCL1 encodes a 114 amino acid residue precursor protein with a 21 amino acid residue predicted signal peptide. The expression of XCL1 is restricted to activated mouse pro-T cells and to activated, class I MHC restricted T cells. The gene for XCL1 has been mapped to chromosome 1 in both human and mouse. Recombinant mouse XCL1 has been shown to have chemotactic activity for lymphocytes, NK cells and mouse splenocytes.
References
- Kelner, G. et al. (1994) Science 266:1395.
- Hedrick, J.A. et al. (1997) J. Immunol. 158:1533.
Long Name
Chemokine [C Motif] Ligand 1
Alternate Names
ATAC, Lymphotactin, SCM-1 alpha
Gene Symbol
XCL1
UniProt
Additional XCL1/Lymphotactin Products
Product Documents for Mouse XCL1/Lymphotactin Antibody
Product Specific Notices for Mouse XCL1/Lymphotactin Antibody
For research use only
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