Mouse XCL1/Lymphotactin Antibody
R&D Systems, part of Bio-Techne | Catalog # AF486
Key Product Details
Species Reactivity
Validated:
Mouse
Cited:
Mouse
Applications
Validated:
Immunohistochemistry, Neutralization, Western Blot
Cited:
ELISA Development, Flow Cytometry, Immunohistochemistry, Neutralization
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Product Specifications
Immunogen
E. coli-derived recombinant mouse XCL1/Lymphotactin
Specificity
Detects XCL1/Lymphotactin in direct ELISAs and Western blots. In direct ELISAs, less than 5% cross-reactivity with recombinant human (rh) GRO alpha, rhGRO beta, rhGRO gamma, and recombinant mouse MCP-5 is observed.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Scientific Data Images for Mouse XCL1/Lymphotactin Antibody
Chemotaxis Induced by XCL1/Lymphotactin and Neutralization by Mouse XCL1/Lymphotactin Antibody.
Recombinant Mouse XCL1/Lymphotactin (Catalog # 486-LT) chemoattracts the BaF3 mouse pro-B cell line transfected with human XCR1 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin. Chemotaxis elicited by Recombinant Mouse XCL1/Lymphotactin (0.15 µg/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Mouse XCL1/ Lymphotactin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF486). The ND50 is typically 5-15 µg/mL.Applications for Mouse XCL1/Lymphotactin Antibody
Application
Recommended Usage
Immunohistochemistry
5-15 µg/mL
Sample: Perfusion fixed frozen sections of mouse small intestine (Peyer's patch) and thymus
Sample: Perfusion fixed frozen sections of mouse small intestine (Peyer's patch) and thymus
Western Blot
0.1 µg/mL
Sample: Recombinant Mouse XCL1/Lymphotactin (Catalog # 486-LT)
Sample: Recombinant Mouse XCL1/Lymphotactin (Catalog # 486-LT)
Neutralization
Measured by its ability to neutralize XCL1/Lymphotactin-induced chemotaxis in the BaF3 mouse pro‑B cell line transfected with human XCR1. The Neutralization Dose (ND50) is typically 5-15 µg/mL in the presence of 0.15 µg/mL Recombinant Mouse XCL1/Lymphotactin.
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: XCL1/Lymphotactin
Mouse lymphotactin (Lptn) and its human homologue (also named human SCM-1 and ATAC) belong to the C or gamma subfamily of chemokines. The C chemokines lack two (the 1st and 3rd) of the four invariant cysteine residues normally found in the CC and CXC chemokines and have an extended carboxy terminus. Mouse lymphotactin encodes a 114 amino acid residue precursor protein with a 21 amino acid residue predicted signal peptide. The expression of lymphotactin is restricted to activated mouse pro-T cells and to activated, class I MHC restricted T cells. The gene for lymphotactin has been mapped to chromosome 1 in both human and mouse.
References
- Kelner, G. et al. (1994) Science 266:1395.
- Hedrick, J.A. et al. (1997) J. Immunol. 158:1533.
Long Name
Chemokine [C Motif] Ligand 1
Alternate Names
ATAC, Lymphotactin, SCM-1 alpha
Gene Symbol
XCL1
Additional XCL1/Lymphotactin Products
Product Documents for Mouse XCL1/Lymphotactin Antibody
Product Specific Notices for Mouse XCL1/Lymphotactin Antibody
For research use only
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