MyoD Antibody (5.8A) [Biotin]

Novus Biologicals, part of Bio-Techne | Catalog # NB100-56511B

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Product Datasheet / COA / SDS

View Available Conjugates View Available Formulations

Conjugate

Catalog #

Alexa Fluor 350

NB100-56511AF350

Alexa Fluor 405

NB100-56511AF405

Alexa Fluor 488

NB100-56511AF488

Alexa Fluor 532

NB100-56511AF532

Alexa Fluor 594

NB100-56511AF594

Alexa Fluor 647

NB100-56511AF647

Alexa Fluor 700

NB100-56511AF700

Alexa Fluor 750

NB100-56511AF750

CoraFluor 1

NB100-56511CL1

DyLight 350

NB100-56511UV

DyLight 405

NB100-56511V

DyLight 488

NB100-56511G

DyLight 550

NB100-56511R

DyLight 594

NB100-56511DL594

DyLight 650

NB100-56511C

DyLight 680

NB100-56511FR

DyLight 755

NB100-56511IR

FITC

NB100-56511F

HRP

NB100-56511H

Janelia Fluor 525

NB100-56511JF525

Janelia Fluor 549

NB100-56511JF549

Janelia Fluor 585

NB100-56511JF585

Janelia Fluor 635

NB100-56511JF635

Janelia Fluor 646

NB100-56511JF646

Janelia Fluor 669

NB100-56511JF669

mFluor Violet 450 SE

NB100-56511MFV450

mFluor Violet 500 SE

NB100-56511MFV500

mFluor Violet 610 SE

NB100-56511MFV610

Forumulation

Catalog #

BSA Free

NB100-56511

Product Summary
Applications
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Species Reactivity

Validated:

Human, Mouse

Predicted:

Porcine (100%), Rat (100%). Backed by our 100% Guarantee.

Applications

Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Immunoprecipitation, Knockdown Validated, Knockout Validated, Western Blot

Label

Biotin

Antibody Source

Monoclonal Mouse IgG₁ kappa Clone # 5.8A

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all MyoD Antibodies »

Product Summary for MyoD Antibody (5.8A) [Biotin]

Immunogen

The 5.8A antibody was made against recombinant mouse MyoD protein but it also recognizes human (myf3), rat, and cat homologs. The epitope of this antibody was mapped to a region within aa 180-189 of mouse MyoD (NP_002469).

Specificity

In Rh-30, a ~45 kDa band should be observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG₁ kappa

Applications for MyoD Antibody (5.8A) [Biotin]

Application

Recommended Usage

Flow Cytometry

Optimal dilutions of this antibody should be experimentally determined.

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry-Frozen

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry-Paraffin

Optimal dilutions of this antibody should be experimentally determined.

Immunoprecipitation

Optimal dilutions of this antibody should be experimentally determined.

Knockdown Validated

Optimal dilutions of this antibody should be experimentally determined.

Knockout Validated

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.

Application Notes

Optimal dilution of this antibody should be experimentally determined.

Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Preservative

0.05% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C in the dark.

Background: MyoD

The Myogenic determination gene (MyoD) was first identified by the virtue of its ability to convert embryonic mouse fibroblast cells to muscle cells. It was subsequently shown that forced expression of MyoD (human homolog is myf 3) gene in a wide variety of normal and neoplastic cells could either convert the cells to muscle cells or activate a set of the otherwise transcriptionally inactive muscle-specific genes in these cells. The regulatory domain of the MyoD gene product lies within a 70 amino acid region and comprises a basic DNA binding motif and a helix-loop-helix (HLH) dimerization motif. Subsequent studies identified three other genes whose products shared sequence homology for the basic HLH domain of MyoD. These are; myf5, myogenin (human homolog is myf4) and myf6 (also known as MRF4 and herculin).

Long Name

Myoblast Determination Protein 1

Alternate Names

bHLHc1, MYF3, MYOD1, PUM

Gene Symbol

MYOD1

Additional MyoD Products

Product Documents for MyoD Antibody (5.8A) [Biotin]

Product Datasheets

Download Product Datasheets

COA

SDS

Product Specific Notices for MyoD Antibody (5.8A) [Biotin]

This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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