Nanog Antibody (CL5810)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-61429

Novus Biologicals, part of Bio-Techne
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NBP2-61429
NBP2-61429-25ul
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Key Product Details

Species Reactivity

Human

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # CL5810

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all Nanog Antibodies »

Product Specifications

Immunogen

This antibody was developed against a synthetic peptide corresponding to amino acids: DSPDSSTSPKGKQPTSAEK

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Nanog Antibody (CL5810)

Western Blot: Nanog Antibody (CL5810) [NBP2-61429]

Western Blot: Nanog Antibody (CL5810) [NBP2-61429]

Western Blot: Nanog Antibody (CL5810) [NBP2-61429] - Analysis in human cell line NTERA-2.
Immunocytochemistry/ Immunofluorescence: Nanog Antibody (CL5810) [NBP2-61429]

Immunocytochemistry/ Immunofluorescence: Nanog Antibody (CL5810) [NBP2-61429]

Immunocytochemistry/Immunofluorescence: Nanog Antibody (CL5810) [NBP2-61429] - Staining of NTERA-2 cells using the Anti-NANOG monoclonal antibody, showing specific staining in the nucleoplasm in green. Microtubule and nuclear probes are visualized in red and blue, respectively (where available).
Immunohistochemistry-Paraffin: Nanog Antibody (CL5810) [NBP2-61429]

Immunohistochemistry-Paraffin: Nanog Antibody (CL5810) [NBP2-61429]

Immunohistochemistry-Paraffin: Nanog Antibody (CL5810) [NBP2-61429] - Staining of human testis shows absence of positivity in seminiferous ducts as expected (negative control).
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Applications for Nanog Antibody (CL5810)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

2-10 ug/ml

Immunohistochemistry

1:1000 - 1:2500

Immunohistochemistry-Paraffin

1:1000 - 1:2500

Western Blot

1 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: PFA/Triton X-100

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Nanog

Nanog is one of the molecular markers suitable for recognizing the undifferentiated state of stem cells in the mouse and human. Nanog is a divergent homeodomain protein that directs propagation of undifferentiated ES cells. Nanog mRNA is present in pluripotent mouse and human cell lines, and absent from differentiated cells. Nanog-deficient ES cells lose pluripotency and differentiate into extraembryonic endoderm lineage.

Long Name

Nanog Homeobox

Alternate Names

FLJ12581, FLJ40451, hNanog, homeobox protein NANOG, Homeobox transcription factor Nanog, homeobox transcription factor Nanog-delta 48, Nanog homeobox

Gene Symbol

NANOG

Additional Nanog Products

Product Documents for Nanog Antibody (CL5810)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Nanog Antibody (CL5810)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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