Nanog Antibody (CL5810) - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-44197

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Human

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # CL5810

Format

Azide and BSA Free

Concentration

LYOPH mg/ml

View all Nanog Antibodies »

Product Specifications

Immunogen

This antibody was generated using a synthetic peptide of Q9H9S0, with the exact immunogen sequence remaining proprietary.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Nanog Antibody (CL5810) - Azide and BSA Free

Nanog Antibody (CL5810) - Azide and BSA Free Immunohistochemistry-Paraffin: Nanog Antibody [NBP3-44197]

Immunohistochemistry-Paraffin: Nanog Antibody [NBP3-44197]

Staining of human renal cancer shows absence of positivity in tumor cells as expected (negative control).
Nanog Antibody (CL5810) - Azide and BSA Free Western Blot: Nanog Antibody [NBP3-44197]

Western Blot: Nanog Antibody [NBP3-44197]

Analysis in human cell line NTERA-2.
Nanog Antibody (CL5810) - Azide and BSA Free Immunocytochemistry/ Immunofluorescence: Nanog Antibody [NBP3-44197]

Immunocytochemistry/ Immunofluorescence: Nanog Antibody [NBP3-44197]

Staining of NTERA-2 cells using the Anti-Nanog monoclonal antibody) .
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Applications for Nanog Antibody (CL5810) - Azide and BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

2-10 ug/ml

Immunohistochemistry-Paraffin

1:1000 - 1:2500

Western Blot

1 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: PFA/Triton X-100

Formulation, Preparation, and Storage

Purification

Protein A purified

Reconstitution

Centrifuge the vial of lyophilized antibody at 12,000 x g for 20 seconds. Reconstitute by adding sterile, distilled water to achieve a final antibody concentration of 1mg/ml.

Formulation

Lyophilized from a 0.2 um filtered solution in PBS with Trehalose

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Nanog

Nanog is one of the molecular markers suitable for recognizing the undifferentiated state of stem cells in the mouse and human. Nanog is a divergent homeodomain protein that directs propagation of undifferentiated ES cells. Nanog mRNA is present in pluripotent mouse and human cell lines, and absent from differentiated cells. Nanog-deficient ES cells lose pluripotency and differentiate into extraembryonic endoderm lineage.

Long Name

Nanog Homeobox

Alternate Names

FLJ12581, FLJ40451, hNanog, homeobox protein NANOG, Homeobox transcription factor Nanog, homeobox transcription factor Nanog-delta 48, Nanog homeobox

Gene Symbol

NANOG

Additional Nanog Products

Product Documents for Nanog Antibody (CL5810) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for Nanog Antibody (CL5810) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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