NCAM-1/CD56 Antibody (CL12732) - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-44370

Novus Biologicals, part of Bio-Techne

Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Human

Applications

Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # CL12732

Format

Azide and BSA Free

Concentration

LYOPH mg/ml

View all NCAM-1/CD56 Antibodies »

Product Specifications

Immunogen

This antibody was generated using a synthetic peptide of P13591, with the exact immunogen sequence remaining proprietary.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Scientific Data Images for NCAM-1/CD56 Antibody (CL12732) - Azide and BSA Free

NCAM-1/CD56 Antibody (CL12732) - Azide and BSA Free Immunohistochemistry-Paraffin: NCAM-1/CD56 Antibody [NBP3-44370]

Immunohistochemistry-Paraffin: NCAM-1/CD56 Antibody [NBP3-44370]

Analysis in human cerebral cortex and skeletal muscle tissues using NBP3-44370 antibody. Corresponding NCAM-1/CD56 RNA-seq data are presented for the same tissues.
NCAM-1/CD56 Antibody (CL12732) - Azide and BSA Free Immunohistochemistry-Paraffin: NCAM-1/CD56 Antibody [NBP3-44370]

Immunohistochemistry-Paraffin: NCAM-1/CD56 Antibody [NBP3-44370]

Staining of human heart shows moderate positivity in the intercalated discs in cardiomyocytes.
NCAM-1/CD56 Antibody (CL12732) - Azide and BSA Free Immunohistochemistry-Paraffin: NCAM-1/CD56 Antibody [NBP3-44370]

Immunohistochemistry-Paraffin: NCAM-1/CD56 Antibody [NBP3-44370]

Staining of human duodenum shows strong positivity in peripheral ganglion and nerves.
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Applications for NCAM-1/CD56 Antibody (CL12732) - Azide and BSA Free

Application
Recommended Usage

Immunohistochemistry-Paraffin

1:5000 - 1:10000
Application Notes
For use with IHC-Paraffin, HIER pH 6 retrieval is recommended.

Formulation, Preparation, and Storage

Purification

Protein A purified

Reconstitution

Centrifuge the vial of lyophilized antibody at 12,000 x g for 20 seconds. Reconstitute by adding sterile, distilled water to achieve a final antibody concentration of 1mg/ml.

Formulation

Lyophilized from a 0.2 um filtered solution in PBS with Trehalose

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: NCAM-1/CD56

NCAM, as a member of the immunoglobulin superfamily of adhesion molecules is characterized by several immunoglobulin (Ig) like domains. The extracellular part of NCAM consists of five of these Ig domains and two fibronectin type III homology regions. NCAM is encoded by a single copy gene composed of 26 exons. However, at least 20-30 distinct isoforms can be generated by alternative splicing and by posttranslational modifications, such as sialylation. During sialylation, polysialic acid (PSA) carbohydrates are attached to the extracellular part of NCAM. Through its extracellular region, NCAM mediates homophilic interactions. In addition, NCAM can also undergo heterophilic interactions by binding extracellular matrix components, such as laminin, or other cell adhesion molecules, such as integrins. NCAM is expressed on most neuroectodermal derived cell lines, tissues and neoplasm such as retinoblastoma, medulloblastoma, astrocytomas and neuroblastoma.

Long Name

Neural Cell Adhesion Molecule

Alternate Names

CD56, NCAM1

Gene Symbol

NCAM1

Additional NCAM-1/CD56 Products

Product Documents for NCAM-1/CD56 Antibody (CL12732) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for NCAM-1/CD56 Antibody (CL12732) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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