Nectin-2/CD112 Antibody (110) - Azide and BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-90530
Recombinant Monoclonal Antibody
![Western Blot: Nectin-2/CD112 Antibody (110) [NBP2-90530]](https://resources.bio-techne.com/images/products/Nectin-2-CD112-Antibody-110-Western-Blot-NBP2-90530-img0003.jpg "Western Blot: Nectin-2/CD112 Antibody (110) [NBP2-90530]")
Conjugate
Catalog #
Key Product Details
Species Reactivity
Mouse
Applications
ELISA, Immunoprecipitation, Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # 110
Format
Azide and BSA Free
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
This antibody was obtained from a rabbit immunized with purified, recombinant Mouse Nectin-2/CD112 (Accession#: NP_033016.3; Met 1-Gly 351).
Specificity
No cross-reactivity in ELISA with: Human CD112 / Nectin-2
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Description
This antibody can be stored at 2C-8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.
Scientific Data Images for Nectin-2/CD112 Antibody (110) - Azide and BSA Free
Western Blot: Nectin-2/CD112 Antibody (110) [NBP2-90530]
Western Blot: Nectin-2/CD112 Antibody (110) [NBP2-90530] - Anti-Carbonic Anhydrase XIV/CA14 rabbit polyclonal antibody at 1:500 dilution. Lane A: Jurkat Membrane Lysate. Lysates/proteins at 20 ug per lane. Secondary Goat Anti-Rabbit IgG H&L (Dylight 800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 38 kDa. Observed band size: 38 kDa![Immunoprecipitation: Nectin-2/CD112 Antibody (110) [NBP2-90530]](https://resources.bio-techne.com/images/products/Nectin-2-CD112-Antibody-110-Immunoprecipitation-NBP2-90530-img0004.jpg "Immunoprecipitation: Nectin-2/CD112 Antibody (110) [NBP2-90530]")
Immunoprecipitation: Nectin-2/CD112 Antibody (110) [NBP2-90530]
Immunoprecipitation: Nectin-2/CD112 Antibody (110) [NBP2-90530] - Mouse Nectin-2/CD112 was immunoprecipitated using: Lane A: 0.5 mg Raji Whole Cell Lysate. 0.5 ul anti-Mouse Nectin-2/CD112 rabbit monoclonal antibody and 15 ul of 50 % Protein G agarose. Primary antibody: Anti-Mouse Nectin-2/CD112 rabbit monoclonal antibody, at 1:500 dilution. Secondary antibody: Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 58 kDa. Observed band size: 58 kDaApplications for Nectin-2/CD112 Antibody (110) - Azide and BSA Free
Application
Recommended Usage
ELISA
1:5000-1:10000
Immunoprecipitation
0.2-1 uL/mg of lysate
Western Blot
1:500-1:1000
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
0.2 um filtered solution in PBS
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: Nectin-2/CD112
Long Name
Poliovirus Receptor Related 2
Alternate Names
CD112, HVEB, MPH, Nectin2, PRR2, PVRL2
Gene Symbol
NECTIN2
Additional Nectin-2/CD112 Products
Product Documents for Nectin-2/CD112 Antibody (110) - Azide and BSA Free
Product Specific Notices for Nectin-2/CD112 Antibody (110) - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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