Nectin-2/CD112 Antibody (JG39-64)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-76852

Recombinant Monoclonal Antibody.
Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Applications

Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # JG39-64

Concentration

1 mg/ml

View all Nectin-2/CD112 Antibodies »

Product Summary for Nectin-2/CD112 Antibody (JG39-64)

Immunogen

Recombinant protein within Human Nectin-2/CD112 aa 370-538 / 538. (SwissProt: Q92692 Human; SwissProt: P32507 Mouse; SwissProt: Q5FVC5 Rat)

Localization

Cell membrane. Membrane.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

58 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Nectin-2/CD112 Antibody (JG39-64)

Western Blot: Nectin-2/CD112 Antibody (JG39-64) [NBP2-76852]

Western Blot: Nectin-2/CD112 Antibody (JG39-64) [NBP2-76852]

Western Blot: Nectin-2/CD112 Antibody (JG39-64) [NBP2-76852] - Analysis of Nectin 2 on K562 (1), MCF-7 (2) and SK-OV-3 (3) cells lysates using anti-Nectin 2 antibody at 1/500 dilution.
Immunocytochemistry/ Immunofluorescence: Nectin-2/CD112 Antibody (JG39-64) [NBP2-76852]

Immunocytochemistry/ Immunofluorescence: Nectin-2/CD112 Antibody (JG39-64) [NBP2-76852]

Immunocytochemistry/Immunofluorescence: Nectin-2/CD112 Antibody (JG39-64) [NBP2-76852] - ICC staining Nectin 2 in LOVO cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Immunohistochemistry: Nectin-2/CD112 Antibody (JG39-64) [NBP2-76852]

Immunohistochemistry: Nectin-2/CD112 Antibody (JG39-64) [NBP2-76852]

Immunohistochemistry: Nectin-2/CD112 Antibody (JG39-64) [NBP2-76852] - Immunohistochemical analysis of paraffin-embedded rat testis tissue using anti-Nectin 2 antibody. Counter stained with hematoxylin.
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Applications for Nectin-2/CD112 Antibody (JG39-64)

Application
Recommended Usage

Flow Cytometry

1:50-1:100

Immunocytochemistry/ Immunofluorescence

1:50

Immunohistochemistry-Paraffin

1:50-1:200

Western Blot

1:500-1:2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

TBS (pH7.4), 0.05% BSA, 40% Glycerol

Preservative

0.05% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Nectin-2/CD112

Nectin 2 encodes a single-pass type I membrane glycoprotein with two Ig-like C2-type domains and an Ig-like V-type domain. This protein is one of the plasma membrane components of adherens junctions. It also serves as an entry for certain mutant strains of herpes simplex virus and pseudorabies virus, and it is involved in cell to cell spreading of these viruses. Variations in this gene have been associated with differences in the severity of multiple sclerosis. Alternate transcriptional splice variants, encoding different isoforms, have been characterized.

Long Name

Poliovirus Receptor Related 2

Alternate Names

CD112, HVEB, MPH, Nectin2, PRR2, PVRL2

Gene Symbol

NECTIN2

Additional Nectin-2/CD112 Products

Product Documents for Nectin-2/CD112 Antibody (JG39-64)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Nectin-2/CD112 Antibody (JG39-64)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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