Nestin Antibody [CoraFluor™ 1]
Novus Biologicals, part of Bio-Techne | Catalog # NB300-265CL1
Conjugate
Catalog #
Key Product Details
Species Reactivity
Validated:
Human, Canine, Mouse (Negative), Rabbit (Negative), Rat (Negative)
Applications
Immunocytochemistry/ Immunofluorescence, Western Blot
Label
CoraFluor 1
Antibody Source
Polyclonal Rabbit IgG
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Summary for Nestin Antibody [CoraFluor™ 1]
Immunogen
Recombinant human Nestin protein (corresponding to residues 1464-1614). [UniProt# P48681]
Reactivity Notes
Canine reactivity reported in scientific literature (PMID: 25088159) Does not cross-react with rodent.
Localization
Cytoplasmic
Marker
Neural Stem Cell Marker
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Description
CoraFluor(TM) 1 is a high performance terbium-based TR-FRET (Time-Resolved Fluorescence Resonance Energy Transfer) or TRF (Time-Resolved Fluorescence) donor for high throughput assay development. CoraFluor(IM) 1 absorbs UV light at approximately 340 nm, and emits at approximately 490 nm, 545 nm, 585 nm and 620 nm. It is compatible with common acceptor dyes that absorb at the emission wavelengths of CoraFluor(TM) 1. CoraFluor(TM) 1 can be used for the development of robust and scalable TR-FRET binding assays such as target engagement, ternary complex, protein-protein interaction and protein quantification assays.
Applications for Nestin Antibody [CoraFluor™ 1]
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
Optimal dilutions of this antibody should be experimentally determined.
Western Blot
Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Optimal dilution of this antibody should be experimentally determined.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
PBS
Preservative
No Preservative
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C in the dark. Do not freeze.
Background: Nestin
Although it is often used as a marker for proliferating and migrating cells very little is known about Nestin's function or regulation.
Alternate Names
NES
Gene Symbol
NES
Additional Nestin Products
Product Documents for Nestin Antibody [CoraFluor™ 1]
Product Specific Notices for Nestin Antibody [CoraFluor™ 1]
CoraFluor (TM) is a trademark of Bio-Techne Corp. Sold for research purposes only under agreement from Massachusetts General Hospital. US patent 2022/0025254
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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