Nestin Antibody [DyLight 680]

Novus Biologicals, part of Bio-Techne | Catalog # NB300-265FR

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Product Datasheet / COA / SDS

View Available Conjugates

Conjugate

Catalog #

Unconjugated

NB300-265

Alexa Fluor 350

NB300-265AF350

Alexa Fluor 405

NB300-265AF405

Alexa Fluor 488

NB300-265AF488

Alexa Fluor 532

NB300-265AF532

Alexa Fluor 594

NB300-265AF594

Alexa Fluor 647

NB300-265AF647

Alexa Fluor 700

NB300-265AF700

Alexa Fluor 750

NB300-265AF750

Allophycocyanin

NB300-265APC

Biotin

NB300-265B

CoraFluor 1

NB300-265CL1

DyLight 350

NB300-265UV

DyLight 405

NB300-265V

DyLight 488

NB300-265G

DyLight 550

NB300-265R

DyLight 594

NB300-265DL594

DyLight 650

NB300-265C

DyLight 755

NB300-265IR

FITC

NB300-265F

HRP

NB300-265H

Janelia Fluor 525

NB300-265JF525

Janelia Fluor 549

NB300-265JF549

Janelia Fluor 585

NB300-265JF585

Janelia Fluor 635

NB300-265JF635

Janelia Fluor 646

NB300-265JF646

Janelia Fluor 669

NB300-265JF669

mFluor Violet 450 SE

NB300-265MFV450

mFluor Violet 500 SE

NB300-265MFV500

mFluor Violet 610 SE

NB300-265MFV610

PE

NB300-265PE

PerCP

NB300-265PCP

Product Summary
Applications
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Species Reactivity

Validated:

Human, Canine, Mouse (Negative), Rabbit (Negative), Rat (Negative)

Applications

Immunocytochemistry/ Immunofluorescence, Western Blot

Label

DyLight 680 (Excitation = 692 nm, Emission = 712 nm)

Antibody Source

Polyclonal Rabbit IgG

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all Nestin Antibodies »

Product Summary for Nestin Antibody [DyLight 680]

Immunogen

Recombinant human Nestin protein (corresponding to residues 1464-1614). [UniProt# P48681]

Reactivity Notes

Canine reactivity reported in scientific literature (PMID: 25088159) Does not cross-react with rodent.

Localization

Cytoplasmic

Marker

Neural Stem Cell Marker

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Applications for Nestin Antibody [DyLight 680]

Application

Recommended Usage

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.

Application Notes

Optimal dilution of this antibody should be experimentally determined.

Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

50mM Sodium Borate

Preservative

0.05% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C in the dark.

Background: Nestin

Nestin is a Class VI intermediate filament expressed in the developing central nervous system in early embryonic neuroepithelial stem cells. This protein has been widely used as a predominant marker for stem / progenitor cells, glioma cells, and tumor endothelial cells. Furthermore, it is a superior angiogenic marker to evaluate neovascularity of endothelial cells in tumor. Because if it's roles in angiogenisis and tumorgenesis, Nestin antibodies are useful cancer markers.

Although it is often used as a marker for proliferating and migrating cells very little is known about Nestin's function or regulation.

Alternate Names

NES

Gene Symbol

NES

Additional Nestin Products

Product Documents for Nestin Antibody [DyLight 680]

Product Datasheets

Download Product Datasheets

COA

SDS

Product Specific Notices for Nestin Antibody [DyLight 680]

DyLight (R) is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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