Neurokinin B Antibody - BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NB300-201
Conjugate
Catalog #
Key Product Details
Validated by
Biological Validation
Species Reactivity
Validated:
Human, Mouse, Rat
Cited:
Human, Mouse, Rat
Applications
Validated:
Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Western Blot
Cited:
IF/IHC, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry-Frozen, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
Concentration
1 mg/ml
Product Specifications
Immunogen
A synthetic peptide made to an internal sequence of mouse proNeurokinin B (~93% homology to the rat sequence). [UniProt# P55099]
Localization
Secreted
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for Neurokinin B Antibody - BSA Free
Immunohistochemistry: Neurokinin B Antibody - BSA Free [NB300-201]
Immunohistochemistry: Neurokinin B Antibody - BSA Free [NB300-201] - Immunofluorescence photomicrograph showing that the expression of Neurokinin B in the arcuate nucleus (ARC). Scale bars: 20 um. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32921318/) licensed under a CC-BY license.Immunohistochemistry-Paraffin: Neurokinin B Antibody - BSA Free [NB300-201]
Immunohistochemistry-Paraffin: Neurokinin B Antibody [NB300-201] - IHC analysis of formalin fixed paraffin embedded tissue section of mouse brain using Neurokinin B antibody at 1:200 dilution. The antibody depicted an expected strong cytoplasmic staining in the neuronal cells.Immunohistochemistry-Paraffin: Neurokinin B Antibody - BSA Free [NB300-201]
Immunohistochemistry-Paraffin: Neurokinin B Antibody [NB300-201] - IHC analysis of formalin fixed paraffin embedded tissue section of mouse brain using Neurokinin B antibody at 1:100 dilution. The antibody showed a specific cytoplasmic staining in the Purkinje cells and some granular neurons from the granular layer with weak signal in the fibers within the molecular layer of the section.Applications for Neurokinin B Antibody - BSA Free
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:10-1:500
Immunohistochemistry
1:100-1:1000
Immunohistochemistry-Frozen
1:500-1:1000
Immunohistochemistry-Paraffin
1:100-1:1000
Western Blot
1:100-1:2000
Application Notes
This Neurokinin B antibody is useful for Immunocytochemistry/Immunofluorescence and Immunohistochemistry on paraffin-embedded and frozen sections. This antibody has been used on human hypothalamus and rat brain sections. In IHC, positive staining of the arcuate nucleus is seen. There has been customer feedback that this antibody works in Western blot; please note that this is a secreted protein.
Reviewed Applications
Read 2 reviews rated 4 using NB300-201 in the following applications:
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
PBS
Format
BSA Free
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Background: Neurokinin B
Alternate Names
gamma tachykinin 3, neurokinin beta, neurokinin B-like, neuromedin K, preprotachykinin B, PRO1155, tachykinin 3, tachykinin-3, ZNEUROK1NKNBNKB
Gene Symbol
TAC3
UniProt
Additional Neurokinin B Products
Product Documents for Neurokinin B Antibody - BSA Free
Product Specific Notices for Neurokinin B Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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