Neurokinin B Antibody - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NB300-201

Novus Biologicals, part of Bio-Techne

Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Human, Mouse, Rat

Applications

Validated:

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Western Blot

Cited:

IF/IHC, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry-Frozen, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

1 mg/ml

View all Neurokinin B Antibodies »

Product Specifications

Immunogen

A synthetic peptide made to an internal sequence of mouse proNeurokinin B (~93% homology to the rat sequence). [UniProt# P55099]

Localization

Secreted

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Neurokinin B Antibody - BSA Free

Immunohistochemistry: Neurokinin B Antibody - BSA Free [NB300-201]

Immunohistochemistry: Neurokinin B Antibody - BSA Free [NB300-201]

Immunohistochemistry: Neurokinin B Antibody - BSA Free [NB300-201] - Immunofluorescence photomicrograph showing that the expression of Neurokinin B in the arcuate nucleus (ARC). Scale bars: 20 um. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32921318/) licensed under a CC-BY license.
Immunohistochemistry-Paraffin: Neurokinin B Antibody - BSA Free [NB300-201]

Immunohistochemistry-Paraffin: Neurokinin B Antibody - BSA Free [NB300-201]

Immunohistochemistry-Paraffin: Neurokinin B Antibody [NB300-201] - IHC analysis of formalin fixed paraffin embedded tissue section of mouse brain using Neurokinin B antibody at 1:200 dilution. The antibody depicted an expected strong cytoplasmic staining in the neuronal cells.
Immunohistochemistry-Paraffin: Neurokinin B Antibody - BSA Free [NB300-201]

Immunohistochemistry-Paraffin: Neurokinin B Antibody - BSA Free [NB300-201]

Immunohistochemistry-Paraffin: Neurokinin B Antibody [NB300-201] - IHC analysis of formalin fixed paraffin embedded tissue section of mouse brain using Neurokinin B antibody at 1:100 dilution. The antibody showed a specific cytoplasmic staining in the Purkinje cells and some granular neurons from the granular layer with weak signal in the fibers within the molecular layer of the section.
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Applications for Neurokinin B Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:10-1:500

Immunohistochemistry

1:100-1:1000

Immunohistochemistry-Frozen

1:500-1:1000

Immunohistochemistry-Paraffin

1:100-1:1000

Western Blot

1:100-1:2000
Application Notes
This Neurokinin B antibody is useful for Immunocytochemistry/Immunofluorescence and Immunohistochemistry on paraffin-embedded and frozen sections. This antibody has been used on human hypothalamus and rat brain sections. In IHC, positive staining of the arcuate nucleus is seen. There has been customer feedback that this antibody works in Western blot; please note that this is a secreted protein.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Reviewed Applications

Read 2 reviews rated 4 using NB300-201 in the following applications:

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: Neurokinin B

Neurokinin B (NKB) belongs to tachykinin family of peptides that also includes substance P, NKA, neuropeptide K, neuropeptide-gamma, and hemokinin-1, and NKB is the only tachykinin synthesized from preprotachykinin-B gene (designated as TAC3 in mammals, except for rodents where it is named Tac2). Tachykinins are characterized by a common carboxyl-terminal amino acid sequence of FXGLM-NH2 , X being a hydrophobic residue and are active peptides which stimulates neurons, evoke behavioral responses, are potent vasodilators and secretagogues (secretion stimulators), and contract directly or indirectly smooth muscles. NKB is the cleaved product of neurokinin B-like protein Zneurok1 (also called preprotachykinin-B or tachykinin-3) and act as an endogenous agonist ligand for its receptor, the NK3 receptor (NKB receptor). NKB signaling has been reported to play critical role in pre-eclampsia which is a potentially life threatening disease and is essential to neuroprotection in neurodegenerative diseases as well as to normal reproductive development.

Alternate Names

gamma tachykinin 3, neurokinin beta, neurokinin B-like, neuromedin K, preprotachykinin B, PRO1155, tachykinin 3, tachykinin-3, ZNEUROK1NKNBNKB

Entrez Gene IDs

6866 (Human); 21334 (Mouse); 29191 (Rat)

Gene Symbol

TAC3

UniProt

P55099 (Mouse)

Additional Neurokinin B Products

Product Documents for Neurokinin B Antibody - BSA Free

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Neurokinin B Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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