Key Product Details

Species Reactivity

Human, Mouse, Rat, Porcine, Bovine, Equine

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # 7D1

Concentration

1 mg/ml

View all NF-L Antibodies »

Product Specifications

Immunogen

Full length native protein purified from pig spinal cord

Marker

Neuronal Marker

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Theoretical MW

68-70 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for NF-L Antibody (7D1)

Western Blot: NF-L Antibody (7D1) [NBP2-50612]

Western Blot: NF-L Antibody (7D1) [NBP2-50612]

Western Blot: NF-L Antibody [NBP2-50612] - Western blot analysis of different tissue lysates using NBP2-50612 to NF-L, MCA-7D1, in green: [1] protein standard (red), [2] rat brain, [3] rat spinal cord, [4] mouse brain, and [5] mouse spinal cord. The strong band at 68kDa corresponds to the NF-L protein.
Immunocytochemistry/ Immunofluorescence: NF-L Antibody (7D1) [NBP2-50612]

Immunocytochemistry/ Immunofluorescence: NF-L Antibody (7D1) [NBP2-50612]

Immunocytochemistry/Immunofluorescence: NF-L Antibody [NBP2-50612] - Immunofluorescent analysis of a section of mouse cerebellum stained with NBP2-50612 in green, and co-stained with a chicken polyclonal antibody to FOX2 in red. The blue is DAPI staining of nuclear DNA. Following transcardial perfusion of mouse with 4% paraformaldehyde, brain was post fixed for 24 hours, cut to 45uM, and free-floating sections were stained with above antibodies. NF-L antibody labels dendrites and axons of neuronal cells predominantly axons in white matter and basket cell axons associated with Purkinje cells. The FOX2 antibody reveals protein expressed in a subset of neurons, including the Purkinje cells.

Applications for NF-L Antibody (7D1)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100 - 1:500

Immunohistochemistry

1:100-1:500

Western Blot

1:5000 - 1:10000
Application Notes
For IHC application, the formalin fixation of the histological sections should be mild in order to achieve a better immunostaining. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

50% PBS, 50% glycerol

Preservative

0.035% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: NF-L

Neuronal intermediate filaments or neurofilaments are found specifically in neurons where they play a key role in maintaining neuronal morphology as well as in regenerating myelinated axons. Neurofilaments contain three subunits: a light polypeptide (NEFL/NFL), a medium polypeptide (NEFM/NFM), and a heavy polypeptide (NEFH/NFH), with molecular weights of 68, 160, and 212 kD respectively. 68kD Neurofilament Light (NEFL) is the most abundant of three neurofilament proteins and, like other nonepithelial intermediate filament proteins, it can form homopolymeric 10-nm filaments. NEFL forms the backbone of neurofilament and perturbations in its expression leads to motor neuron diseases, such as Charcot-Marie-Tooth disease, type 2E (CMT2E). In addition to its influence on the nervous system, NEFL has been shown to act as a tumor suppressor and has been linked to carcinogenesis/metastasis of several malignancues including breast, prostate, lung, colon, and bladder cancer.

Long Name

Neurofilament Protein, Light Chain

Alternate Names

CMT2E, NEFL, NF68, NFL

Entrez Gene IDs

18039 (Mouse)

Gene Symbol

NEFL

Additional NF-L Products

Product Documents for NF-L Antibody (7D1)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for NF-L Antibody (7D1)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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