NF-L Antibody (CL4729) - Azide and BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-44128
![NF-L Antibody (CL4729) - Azide and BSA Free Immunocytochemistry/ Immunofluorescence: NF-L Antibody [NBP3-44128]](https://resources.bio-techne.com/images/products/nbp3-44128_mouse-nf-l-mab-cl4729-azide-and-bsa-free-282202513381527.jpg "Immunocytochemistry/ Immunofluorescence: NF-L Antibody [NBP3-44128]")
![NF-L Antibody (CL4729) - Azide and BSA Free Immunohistochemistry-Paraffin: NF-L Antibody [NBP3-44128]](https://resources.bio-techne.com/images/products/nbp3-44128_mouse-nf-l-mab-cl4729-azide-and-bsa-free-282202512154149.jpg "Immunohistochemistry-Paraffin: NF-L Antibody [NBP3-44128]")
Conjugate
Catalog #
Forumulation
Catalog #
Key Product Details
Species Reactivity
Human, Mouse, Rat
Applications
Immunocytochemistry/ Immunofluorescence, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # CL4729
Format
Azide and BSA Free
Concentration
LYOPH mg/ml
Product Specifications
Immunogen
This antibody was generated using a recombinant protein sequence of P07196, with the exact immunogen sequence remaining proprietary.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Scientific Data Images for NF-L Antibody (CL4729) - Azide and BSA Free
Immunohistochemistry-Paraffin: NF-L Antibody [NBP3-44128]
Staining of human cerebellum shows strong cytoplasmic immunoreactivity in cell bodies and proximal dendrites of Purkinje cells.![NF-L Antibody (CL4729) - Azide and BSA Free Immunohistochemistry-Paraffin: NF-L Antibody [NBP3-44128]](https://resources.bio-techne.com/images/products/nbp3-44128_mouse-nf-l-mab-cl4729-azide-and-bsa-free-282202512303839.jpg "Immunohistochemistry-Paraffin: NF-L Antibody [NBP3-44128]")
Immunohistochemistry-Paraffin: NF-L Antibody [NBP3-44128]
Staining of human liver shows no positivity as expected (negative control).![NF-L Antibody (CL4729) - Azide and BSA Free Immunohistochemistry-Paraffin: NF-L Antibody [NBP3-44128]](https://resources.bio-techne.com/images/products/nbp3-44128_mouse-nf-l-mab-cl4729-azide-and-bsa-free-282202512295624.jpg "Immunohistochemistry-Paraffin: NF-L Antibody [NBP3-44128]")
Immunohistochemistry-Paraffin: NF-L Antibody [NBP3-44128]
Staining of human cerebral cortex shows strong positivity in neural fibers.![NF-L Antibody (CL4729) - Azide and BSA Free Immunohistochemistry-Paraffin: NF-L Antibody [NBP3-44128]](https://resources.bio-techne.com/images/products/nbp3-44128_mouse-nf-l-mab-cl4729-azide-and-bsa-free-282202513261972.jpg "Immunohistochemistry-Paraffin: NF-L Antibody [NBP3-44128]")
![NF-L Antibody (CL4729) - Azide and BSA Free Western Blot: NF-L Antibody [NBP3-44128]](https://resources.bio-techne.com/images/products/nbp3-44128_mouse-nf-l-mab-cl4729-azide-and-bsa-free-282202513355878.jpg "Western Blot: NF-L Antibody [NBP3-44128]")
![NF-L Antibody (CL4729) - Azide and BSA Free Immunohistochemistry-Paraffin: NF-L Antibody [NBP3-44128]](https://resources.bio-techne.com/images/products/nbp3-44128_mouse-nf-l-mab-cl4729-azide-and-bsa-free-282202513401732.jpg "Immunohistochemistry-Paraffin: NF-L Antibody [NBP3-44128]")
![NF-L Antibody (CL4729) - Azide and BSA Free Immunohistochemistry-Paraffin: NF-L Antibody [NBP3-44128]](https://resources.bio-techne.com/images/products/nbp3-44128_mouse-nf-l-mab-cl4729-azide-and-bsa-free-282202513415610.jpg "Immunohistochemistry-Paraffin: NF-L Antibody [NBP3-44128]")
![NF-L Antibody (CL4729) - Azide and BSA Free Immunohistochemistry-Paraffin: NF-L Antibody [NBP3-44128]](https://resources.bio-techne.com/images/products/nbp3-44128_mouse-nf-l-mab-cl4729-azide-and-bsa-free-2822025133783.jpg "Immunohistochemistry-Paraffin: NF-L Antibody [NBP3-44128]")
Applications for NF-L Antibody (CL4729) - Azide and BSA Free
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
2-10 ug/ml
Immunohistochemistry-Paraffin
1:10000 - 1:20000
Western Blot
1 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/ Immunofluorescence/IF Fixation/Permeabilization: PFA/Triton X-100.
Formulation, Preparation, and Storage
Purification
Protein A purified
Reconstitution
Centrifuge the vial of lyophilized antibody at 12,000 x g for 20 seconds. Reconstitute by adding sterile, distilled water to achieve a final antibody concentration of 1mg/ml.
Formulation
Lyophilized from a 0.2 um filtered solution in PBS with Trehalose
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
LYOPH mg/ml
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: NF-L
Antibodies to NF-L may be used to identify NF-L in neurons and to study neuronal processes in tissue sections and/or culture. NF-L antibodies can also be used to study microfilament accumulations seen in many neurological diseases, such as Lou Geri's disease or Alzheimer's disease.
Long Name
Neurofilament Protein, Light Chain
Alternate Names
CMT2E, NEFL, NF68, NFL
Gene Symbol
NEFL
Additional NF-L Products
Product Documents for NF-L Antibody (CL4729) - Azide and BSA Free
Product Specific Notices for NF-L Antibody (CL4729) - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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