NF-L Antibody (CL4729) - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-44128

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Product Datasheet / COA

View Available Conjugates

Conjugate

Catalog #

Unconjugated

NBP2-59061

Alexa Fluor 350

NBP2-59061AF350

Alexa Fluor 405

NBP2-59061AF405

Alexa Fluor 488

NBP2-59061AF488

Alexa Fluor 532

NBP2-59061AF532

Alexa Fluor 594

NBP2-59061AF594

Alexa Fluor 647

NBP2-59061AF647

Alexa Fluor 700

NBP2-59061AF700

Alexa Fluor 750

NBP2-59061AF750

Allophycocyanin

NBP2-59061APC

Biotin

NBP2-59061B

CoraFluor 1

NBP2-59061CL1

DyLight 350

NBP2-59061UV

DyLight 405

NBP2-59061V

DyLight 488

NBP2-59061G

DyLight 550

NBP2-59061R

DyLight 594

NBP2-59061DL594

DyLight 650

NBP2-59061C

DyLight 680

NBP2-59061FR

DyLight 755

NBP2-59061IR

FITC

NBP2-59061F

HRP

NBP2-59061H

Janelia Fluor 525

NBP2-59061JF525

Janelia Fluor 549

NBP2-59061JF549

Janelia Fluor 585

NBP2-59061JF585

Janelia Fluor 635

NBP2-59061JF635

Janelia Fluor 646

NBP2-59061JF646

Janelia Fluor 669

NBP2-59061JF669

mFluor Violet 450 SE

NBP2-59061MFV450

mFluor Violet 500 SE

NBP2-59061MFV500

mFluor Violet 610 SE

NBP2-59061MFV610

PE

NBP2-59061PE

PerCP

NBP2-59061PCP

Product Specifications
Applications
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Mouse IgG₁ Clone # CL4729

Format

Azide and BSA Free

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all NF-L Antibodies »

Product Specifications

Immunogen

This antibody was generated using a recombinant protein sequence of P07196, with the exact immunogen sequence remaining proprietary.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG₁

Applications for NF-L Antibody (CL4729) - Azide and BSA Free

Application

Recommended Usage

Immunocytochemistry/ Immunofluorescence

: 2-10 ug/ml

Immunohistochemistry

: 1:10000 - 1:20000

Immunohistochemistry-Paraffin

: 1:10000 - 1:20000

Western Blot

: 1 ug/ml

Application Notes

For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/ Immunofluorescence/IF Fixation/Permeabilization: PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

Lyophilized in 5% Trehalose.

Format

Azide and BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: NF-L

Neurofilaments are 10nm intermediate filament proteins located in vertebrate neurons, which regulate axonal diameter. They are composed predominantly of the three major neurofilament proteins: NF-Light, NF-Medium and NF-Heavy. NF-L is the light or low molecular weight polypeptide, and it runs on SDS-PAGE gels at about 68kDa, with some size variation in across species.

Antibodies to NF-L may be used to identify NF-L in neurons and to study neuronal processes in tissue sections and/or culture. NF-L antibodies can also be used to study microfilament accumulations seen in many neurological diseases, such as Lou Geri's disease or Alzheimer's disease.

Long Name

Neurofilament Protein, Light Chain

Alternate Names

CMT2E, NEFL, NF68, NFL

Gene Symbol

NEFL

Additional NF-L Products

Product Documents for NF-L Antibody (CL4729) - Azide and BSA Free

Product Datasheets

Download Product Datasheets

COA

Product Specific Notices for NF-L Antibody (CL4729) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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