NIFK Antibody (CL2240)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-36749

Novus Biologicals, part of Bio-Techne
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NBP2-36749
NBP2-36749-25ul
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Key Product Details

Species Reactivity

Human

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # CL2240

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all NIFK Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids:IDYDFPSLILQKTESISKTNRQTSTKGQVLRKKKKKVSGTLDTPEKTVDSQGPTPVCTPTFLERRKSQVAELNDDDKDDEI

Epitope

KVSGTLDTPE

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Scientific Data Images for NIFK Antibody (CL2240)

Immunocytochemistry/ Immunofluorescence: NIFK Antibody (CL2240) [NBP2-36749]

Immunocytochemistry/ Immunofluorescence: NIFK Antibody (CL2240) [NBP2-36749]

Immunocytochemistry/Immunofluorescence: NIFK Antibody (CL2240) [NBP2-36749] - Staining in U251 cell line showing specific staining of nucleoli in green. Microtubule-staining and nuclear probes are visualized in red and blue respectively (where available). Antibody staining is shown in green.
Immunohistochemistry: NIFK Antibody (CL2240) [NBP2-36749]

Immunohistochemistry: NIFK Antibody (CL2240) [NBP2-36749]

Immunohistochemistry: NIFK Antibody (CL2240) [NBP2-36749] - Staining of human kidney shows strong nucleolar positivity in renal tubules.
Immunocytochemistry/ Immunofluorescence: NIFK Antibody (CL2240) [NBP2-36749]

Immunocytochemistry/ Immunofluorescence: NIFK Antibody (CL2240) [NBP2-36749]

Immunocytochemistry/Immunofluorescence: NIFK Antibody (CL2240) [NBP2-36749] - Staining in HeLa cell line showing specific staining of nucleoli in green. Microtubule-staining and nuclear probes are visualized in red and blue respectively (where available). Antibody staining is shown in green.
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Applications for NIFK Antibody (CL2240)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

2-10 ug/ml

Immunohistochemistry

1:200 - 1:500

Immunohistochemistry-Paraffin

1:200 - 1:500
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: NIFK

NIFK is a nucleolar protein that interacts with Ki67, a protein strongly associated with, and a specific marker of, cell proliferation. The marsupial counterpart of human NIFK was recently identified and suggests that NIFK may be involved in the organization of higher order chromatin structure. NIFK was found to interact with the forkhead associated domain of Ki67 in mitotic cells and the interaction is thought to be phosphorylation-dependent.

Alternate Names

hNIFK, MKI67 (FHA domain) interacting nucleolar phosphoprotein, MKI67 FHA domain-interacting nucleolar phosphoprotein, NIFKNOPP34, Nopp34, Nucleolar phosphoprotein Nopp34, Nucleolar protein interacting with the FHA domain of pKI-67

Gene Symbol

NIFK

UniProt

Q9BYG3 (Human)

Additional NIFK Products

Product Documents for NIFK Antibody (CL2240)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for NIFK Antibody (CL2240)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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