NIFK Antibody (CL2240) - Azide and BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-44459
![NIFK Antibody (CL2240) - Azide and BSA Free Immunocytochemistry/ Immunofluorescence: NIFK Antibody [NBP3-44459]](https://resources.bio-techne.com/images/products/nbp3-44459_mouse-nifk-mab-cl2240-azide-and-bsa-free-28220251211533.jpg "Immunocytochemistry/ Immunofluorescence: NIFK Antibody [NBP3-44459]")
![NIFK Antibody (CL2240) - Azide and BSA Free Immunocytochemistry/ Immunofluorescence: NIFK Antibody [NBP3-44459]](https://resources.bio-techne.com/images/products/nbp3-44459_mouse-nifk-mab-cl2240-azide-and-bsa-free-282202512173182.jpg "Immunocytochemistry/ Immunofluorescence: NIFK Antibody [NBP3-44459]")
Conjugate
Catalog #
Forumulation
Catalog #
Key Product Details
Species Reactivity
Human
Applications
Immunocytochemistry/ Immunofluorescence, Immunohistochemistry-Paraffin
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2A Clone # CL2240
Format
Azide and BSA Free
Concentration
LYOPH mg/ml
Product Specifications
Immunogen
This antibody was generated using a recombinant protein sequence of Q9BYG3, with the exact immunogen sequence remaining proprietary.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2A
Scientific Data Images for NIFK Antibody (CL2240) - Azide and BSA Free
Immunocytochemistry/ Immunofluorescence: NIFK Antibody [NBP3-44459]
Staining in MCF7 cell line with Anti-MKI67IP monoclonal antibody) .![NIFK Antibody (CL2240) - Azide and BSA Free Immunocytochemistry/ Immunofluorescence: NIFK Antibody [NBP3-44459]](https://resources.bio-techne.com/images/products/nbp3-44459_mouse-nifk-mab-cl2240-azide-and-bsa-free-28220251283724.jpg "Immunocytochemistry/ Immunofluorescence: NIFK Antibody [NBP3-44459]")
Immunocytochemistry/ Immunofluorescence: NIFK Antibody [NBP3-44459]
Staining in U2OS cell line with Anti-MKI67IP monoclonal antibody) .![NIFK Antibody (CL2240) - Azide and BSA Free Immunocytochemistry/ Immunofluorescence: NIFK Antibody [NBP3-44459]](https://resources.bio-techne.com/images/products/nbp3-44459_mouse-nifk-mab-cl2240-azide-and-bsa-free-282202512121915.jpg "Immunocytochemistry/ Immunofluorescence: NIFK Antibody [NBP3-44459]")
Immunocytochemistry/ Immunofluorescence: NIFK Antibody [NBP3-44459]
Staining in U251 cell line with Anti-MKI67IP monoclonal antibody) .![NIFK Antibody (CL2240) - Azide and BSA Free Immunocytochemistry/ Immunofluorescence: NIFK Antibody [NBP3-44459]](https://resources.bio-techne.com/images/products/nbp3-44459_mouse-nifk-mab-cl2240-azide-and-bsa-free-282202512104215.jpg "Immunocytochemistry/ Immunofluorescence: NIFK Antibody [NBP3-44459]")
![NIFK Antibody (CL2240) - Azide and BSA Free Immunohistochemistry-Paraffin: NIFK Antibody [NBP3-44459]](https://resources.bio-techne.com/images/products/nbp3-44459_mouse-nifk-mab-cl2240-azide-and-bsa-free-28220251235194.jpg "Immunohistochemistry-Paraffin: NIFK Antibody [NBP3-44459]")
![NIFK Antibody (CL2240) - Azide and BSA Free Immunohistochemistry-Paraffin: NIFK Antibody [NBP3-44459]](https://resources.bio-techne.com/images/products/nbp3-44459_mouse-nifk-mab-cl2240-azide-and-bsa-free-282202512321074.jpg "Immunohistochemistry-Paraffin: NIFK Antibody [NBP3-44459]")
![NIFK Antibody (CL2240) - Azide and BSA Free Immunohistochemistry-Paraffin: NIFK Antibody [NBP3-44459]](https://resources.bio-techne.com/images/products/nbp3-44459_mouse-nifk-mab-cl2240-azide-and-bsa-free-282202512394491.jpg "Immunohistochemistry-Paraffin: NIFK Antibody [NBP3-44459]")
![NIFK Antibody (CL2240) - Azide and BSA Free Immunohistochemistry-Paraffin: NIFK Antibody [NBP3-44459]](https://resources.bio-techne.com/images/products/nbp3-44459_mouse-nifk-mab-cl2240-azide-and-bsa-free-28220251331489.jpg "Immunohistochemistry-Paraffin: NIFK Antibody [NBP3-44459]")
Applications for NIFK Antibody (CL2240) - Azide and BSA Free
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
2-10 ug/ml
Immunohistochemistry-Paraffin
1:200 - 1:500
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/ Immunofluorescence/IF Fixation Permeabilization: Use PFA/Triton X-100.
Formulation, Preparation, and Storage
Purification
Protein A purified
Reconstitution
Centrifuge the vial of lyophilized antibody at 12,000 x g for 20 seconds. Reconstitute by adding sterile, distilled water to achieve a final antibody concentration of 1mg/ml.
Formulation
Lyophilized from a 0.2 um filtered solution in PBS with Trehalose
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
LYOPH mg/ml
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: NIFK
Alternate Names
hNIFK, MKI67 (FHA domain) interacting nucleolar phosphoprotein, MKI67 FHA domain-interacting nucleolar phosphoprotein, NIFKNOPP34, Nopp34, Nucleolar phosphoprotein Nopp34, Nucleolar protein interacting with the FHA domain of pKI-67
Gene Symbol
NIFK
Additional NIFK Products
Product Documents for NIFK Antibody (CL2240) - Azide and BSA Free
Product Specific Notices for NIFK Antibody (CL2240) - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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