Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # OTI1H8

Concentration

1 mg/ml

View all Noggin Antibodies »

Product Specifications

Immunogen

Human recombinant protein fragment corresponding to amino acids 28-232 of human noggin (NP_005441) produced in E.coli.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Specificity

This antibody is specific for Homo sapiens noggin (NOG).

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Theoretical MW

23.7 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Noggin Antibody (OTI1H8)

Western Blot: Noggin Antibody (OTI1H8) [NBP1-47881]

Western Blot: Noggin Antibody (OTI1H8) [NBP1-47881]

Western Blot: Noggin Antibody (OTI1H8) [NBP1-47881] - Noggin Antibody (1H8) [NBP1-47881] - Analysis of extracts (35ug) from 9 different cell lines by using anti-Noggin monoclonal antibody.
Immunocytochemistry/ Immunofluorescence: Noggin Antibody (OTI1H8) [NBP1-47881]

Immunocytochemistry/ Immunofluorescence: Noggin Antibody (OTI1H8) [NBP1-47881]

Immunocytochemistry/Immunofluorescence: Noggin Antibody (OTI1H8) [NBP1-47881] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY Noggin.
Immunohistochemistry-Paraffin: Noggin Antibody (OTI1H8) [NBP1-47881]

Immunohistochemistry-Paraffin: Noggin Antibody (OTI1H8) [NBP1-47881]

Immunohistochemistry-Paraffin: Noggin Antibody (1H8) [NBP1-47881] - Staining of paraffin-embedded Human Ovary tissue using anti-Noggin mouse monoclonal antibody.
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Applications for Noggin Antibody (OTI1H8)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100

Immunohistochemistry

1:50

Immunohistochemistry-Paraffin

1:50

Western Blot

1:20000-40000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.3), 1.0% BSA and 50% Glycerol

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Noggin

Noggin is a secreted polypeptide which binds and inactivates members of the transforming growth factor-beta (TGF-beta) superfamily signaling proteins, such as bone morphogenetic protein-4 (BMP4). By diffusing through extracellular matrices more efficiently than members of the TGF-beta superfamily, it may have a principal role in creating morphogenic gradients. Noggin appears to have pleiotropic effect, both early in development as well as in later stages. The results of the mouse knockout of the ortholog suggest that noggin is involved in numerous developmental processes, such as neural tube fusion and joint formation. Recently, several dominant human NOG mutations in unrelated families with proximal symphalangism (SYM1) and multiple synostoses syndrome (SYNS1) were identified; both SYM1 and SYNS1 have multiple joint fusion as their principal feature, and map to the same region (17q22) as this gene. All of these mutations altered evolutionarily conserved amino acid residues. The amino acid sequence of this human gene is highly homologous to that of Xenopus, rat and mouse

Alternate Names

NOG, SYM1, SYNS1, SYNS1A

Entrez Gene IDs

9241 (Human)

Gene Symbol

NOG

Additional Noggin Products

Product Documents for Noggin Antibody (OTI1H8)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for Noggin Antibody (OTI1H8)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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