Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # OTI1H8

Format

Azide and BSA Free

Concentration

LYOPH mg/ml

View all Noggin Antibodies »

Product Specifications

Immunogen

Human recombinant protein fragment corresponding to amino acids 28-232 of human noggin (NP_005441) produced in E.coli.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Theoretical MW

23.7 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Noggin Antibody (OTI1H8) - Azide and BSA Free

Western Blot: Noggin Antibody (OTI1H8)Azide and BSA Free [NBP2-73030]

Western Blot: Noggin Antibody (OTI1H8)Azide and BSA Free [NBP2-73030]

Western Blot: Noggin Antibody (OTI1H8) - Azide and BSA Free [NBP2-73030] - Noggingin antibody (1H8) at 1:40000 + Recombinant human Noggingin.
Immunocytochemistry/ Immunofluorescence: Noggin Antibody (OTI1H8) - Azide and BSA Free [NBP2-73030]

Immunocytochemistry/ Immunofluorescence: Noggin Antibody (OTI1H8) - Azide and BSA Free [NBP2-73030]

Immunocytochemistry/Immunofluorescence: Noggin Antibody (OTI1H8) - Azide and BSA Free [NBP2-73030] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY Noggin.
Immunohistochemistry: Noggin Antibody (OTI1H8) - Azide and BSA Free [NBP2-73030]

Immunohistochemistry: Noggin Antibody (OTI1H8) - Azide and BSA Free [NBP2-73030]

Immunohistochemistry: Noggin Antibody (OTI1H8) - Azide and BSA Free [NBP2-73030] - Staining of paraffin-embedded Human lung tissue using anti-Noggin mouse monoclonal antibody.
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Applications for Noggin Antibody (OTI1H8) - Azide and BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100

Immunohistochemistry

1:50

Western Blot

1:20000-40000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Reconstitution

we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Formulation

Lyophilized from PBS (pH 7.3) with 8% Trehalose

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Noggin

Noggin is involved in numerous developmental processes, such as neural tube fusion and joint formation. The morphogenesis of organs is initiated by a downgrowth from a layer of epithelial stem cells. This process is achieved through the receipt of signals from 1) a WNT protein (WNT3A) to stabilize beta-catenin; and 2) Noggin, which is a bone morphogenetic protein inhibitor. Noggin mutations in unrelated families with proximal symphalangism (SYM1) and multiple synostoses syndrome (SYNS1) have been identified, which have multiple joint fusion as their principal defect.

Alternate Names

NOG, SYM1, SYNS1, SYNS1A

Gene Symbol

NOG

Additional Noggin Products

Product Documents for Noggin Antibody (OTI1H8) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for Noggin Antibody (OTI1H8) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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