Noxa Antibody (114C307.1) - Non-Recombinant Monoclonal

Flow Cytometry: Noxa Antibody (114C307.1) - Non-Recombinant Monoclonal [NB600-1159]

Flow Cytometry: Noxa Antibody (114C307.1) - Non-Recombinant Monoclonal [NB600-1159] - Flow cytometry after an intracellular stain was performed on MCF7 cells with Noxa [114C307.1] antibody NB600-1159AF700 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 700.

Western Blot: Noxa Antibody (114C307.1)Non-Recombinant Monoclonal [NB600-1159]

Western Blot: Noxa Antibody (114C307.1) - Non-Recombinant Monoclonal [NB600-1159] - Doc counteracts the ABT-737-mediated increase in Mcl-1 protein. Western blot showing treatment of LNCaP and PC3 cells with 1 uM ABT-737. In LNCaP and PC3, there are few difference in Noxa. Image collected and cropped by CiteAb from the following publication (https://peerj.com/articles/144), licensed under a CC-BY license.

Flow Cytometry: Noxa Antibody (114C307.1) - Non-Recombinant Monoclonal [NB600-1159]

Flow Cytometry: Noxa Antibody (114C307.1) - Non-Recombinant Monoclonal [NB600-1159] - Flow cytometry after an intracellular stain was performed on HeLa cells with Noxa [114C307.1] antibody NB600-1159AF700 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 700.

Flow Cytometry: Noxa Antibody (114C307.1) - Non-Recombinant Monoclonal [NB600-1159]

Flow Cytometry: Noxa Antibody (114C307.1) - Non-Recombinant Monoclonal [NB600-1159] - Flow cytometry after an intracellular stain was performed on MCF7 cells with Noxa [114C307.1] antibody NB600-1159AF700 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 700.

Flow Cytometry: Noxa Antibody (114C307.1) - Non-Recombinant Monoclonal [NB600-1159]

Flow Cytometry: Noxa Antibody (114C307.1) - Non-Recombinant Monoclonal [NB600-1159] - An intracellular stain was performed on Ntera2 cells with Noxa Antibody [114C307.1] NB600-1159 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 1.0 ug/mL for 30 minutes at room temperature, followed by Mouse IgG (H+L) Cross-Adsorbed Secondary Antibody, Dylight 550 (35503, Thermo Fisher).

Western Blot: Noxa Antibody (114C307.1) - Non-Recombinant Monoclonal [NB600-1159] -

Doc counteracts the ABT-737-mediated increase in Mcl-1 protein.Western blot showing that treatment of LNCaP and PC3 cells with 1 µM ABT-737 (A) increases Mcl-1 but treatment with 1 nM Doc (D) decreases Mcl-1. Combination of Doc + ABT-737 (DA) decreases Mcl-1. In LNCaP, there is less Bcl-2 (D, 24 h) and Bak (DA, 48 h) but few differences in Bcl-xL, Bax, Bid, and Noxa. In PC3, there are few differences in Bcl-2, Bcl-xL, Bax, Bak, Bid, and Noxa.

Western Blot: Noxa Antibody (114C307.1) - Non-Recombinant Monoclonal [NB600-1159] -

Western Blot: Noxa Antibody (114C307.1) - Non-Recombinant Monoclonal [NB600-1159] - EGb761 regulates Bcl-2 family proteins expression in melanoma cells.(A) EGb761 alters the expression levels of anti- & pro-apoptotic Bcl-2 family proteins in melanoma cell lines. Whole cell lysates from Mel-RM & Mel-AT cells treated with EGb761 (400 μg/ml) for indicated time periods were subjected to Western blot analysis. The data shown are representative of three individual experiments. (B) 5% ethanol as control vehicle did not alter the expression levels of Mcl-1. Mel-AT cells with 5% ethanol for increasing periods. Whole cell lysates from Mel-AT cells treated were subjected to Western blot analysis. The data shown are representative of three individual experiments.(C) Mel-RM & Mel-AT cells were treated with EGb761 (400 μg/ml) or 5% ethanol for the indicated periods. Total RNA was isolated & subjected to real-time PCR analysis for Mcl-1. The relative abundance of mRNA expression treated with 5% ethanol was arbitrarily designated as 1. Columns, mean of three individual experiments; bars, SEM. * Present p<0.05 vs control.(D) Relative expression of anti-apoptosis Bcl-2 family proteins in melanoma cell lines Mel-RM & Mel-AT without treatment. Quantitative expression levels of Mcl-1, Bcl-2 & Bcl-XL were normalized to GAPDH.(E) Relative expression of pro-apoptosis Bcl-2 family proteins in melanoma cell lines Mel-RM & Mel-AT without treatment. Quantitative expression levels of Bax, Bid, Noxa, PUMA, Bim, Bad & Bak were normalized to GAPDH. Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/25860257), licensed under a CC-BY license. Not internally tested by Novus Biologicals.