Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Applications

Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 114C307.1

Format

Azide Free

Concentration

1.0 mg/ml

View all Noxa Antibodies »

Product Summary for Noxa Antibody (114C307.1) - Azide Free

Immunogen

This Noxa antibody was developed by immunizing mice with a fusion protein containing human Noxa.

Reactivity Notes

Reactivity with Rat is reported in scientific literature (Seda et al Hepatol Res. 2010, 40: 701-710).

Localization

Mitochondrion, also reported in Cytosol (PMID 21145489) & Nucleus (PMID 12879012).

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Theoretical MW

14.96 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Noxa Antibody (114C307.1) - Azide Free

Western Blot: Noxa Antibody (114C307.1)Azide Free [NBP2-33250]

Western Blot: Noxa Antibody (114C307.1)Azide Free [NBP2-33250]

Western Blot: Noxa Antibody (114C307.1) - Azide Free [NBP2-33250] - Detection by Western blot in RL-7 cells (a follicular lymphoma).
Immunocytochemistry/ Immunofluorescence: Noxa Antibody (114C307.1) - Azide Free [NBP2-33250]

Immunocytochemistry/ Immunofluorescence: Noxa Antibody (114C307.1) - Azide Free [NBP2-33250]

Immunocytochemistry/Immunofluorescence: Noxa Antibody (114C307.1) - Azide Free [NBP2-33250] - ICC/IF analysis in HeLa cells with DyLight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and DyLight 550 (red). An antibody dilution of 1:10 was used. Image objective 40x.
Immunohistochemistry-Paraffin: Noxa Antibody (114C307.1) - Azide Free [NBP2-33250]

Immunohistochemistry-Paraffin: Noxa Antibody (114C307.1) - Azide Free [NBP2-33250]

Immunohistochemistry-Paraffin: Noxa Antibody (114C307.1) - Azide Free [NBP2-33250] - Immunohistochemical analysis of bouin-fixed, paraffin-embedded chronic lymphocytic leukemia (CLL) xenograft stained with Noxa antibody (1:2000), peroxidase-conjugate and DAB chromogen. Staining for 2 hr at RT was used.
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Applications for Noxa Antibody (114C307.1) - Azide Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

reported in scientific literature (Yakovlev et al (2003))

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Paraffin

5 ug/ml

Western Blot

1-2 ug/ml
Application Notes
An ~80 kDa background band may also be seen in Jurkat cell lysate.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS - BSA free

Format

Azide Free

Preservative

No Preservative

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Noxa

Noxa, phorbol-12-myristate-13-acetate-induced protein 1, PMA-induced protein 1, PMAIP1 (Human 6 kDa and Mouse 11.6 kDa) is a Bcl2 homology 3 (BH3) domain containing protein which plays a role in the regulation of apoptosis, specifically a pro-apoptotic role. Human Noxa contains only one BH3 domain, while the mouse and rat proteins contain two BH3 domains. The human protein is encoded by Exon 1 and 3, with Exon 2 present only in two splice variants. Human Noxa splice variants lack a BH3 domain, have no known function, and are rapidly targeted for degradation (1).

Noxa mediated apoptosis may follow its transcriptional activation by p53 as part of the DNA damage response. However, Noxa expression may be induced by HIF-1 alpha under hypoxia, promoting apoptosis independently of p53 (1). Inhibition of the anti-apoptotic Bcl2 family member Myeloid cell leukemia-1 (Mcl-1) by Noxa, leads to the activation of pro-apoptotic Bcl2 homologous antagonist killer (Bak) and Bcl2 associated X (Bax) proteins, and apoptosis through the intrinsic mitochondrial pathway (2). Other BH3 only proteins such as Bim, Puma, Bad and Bid act via the same mechanism, targeting Mcl-1 and inducing Bak/Bax mediated mitochondrial outer membrane permeabilization (MOMP), cytochrome c release and caspase 9 activation (3). Overexpression of antiapoptotic Bcl2 family members is common in various types of cancer including prostate cancer. A recent study identified antiapoptotic Bcl2 proteins involved in the development of resistance towards the androgen receptor antagonist enzalutamide, which is used for the treatment of metastatic castration-resistant prostate cancer (mCRPC) (3).

References

1.Ploner, C., Kofler, R., & Villunger, A. (2008). Noxa: At the tip of the balance between life and death. Oncogene. https://doi.org/10.1038/onc.2009.46

2.Xiang, W., Yang, C. Y., & Bai, L. (2018). MCL-1 inhibition in cancer treatment. OncoTargets and Therapy. https://doi.org/10.2147/OTT.S146228

3.Pilling, A. B., & Hwang, C. (2019). Targeting prosurvival BCL2 signaling through Akt blockade sensitizes castration-resistant prostate cancer cells to enzalutamide. Prostate. https://doi.org/10.1002/pros.23843

Alternate Names

adult T cell leukemia-derived PMA-responsive, APR, Immediate-early-response protein APR, NOXAPMA-induced protein 1, phorbol-12-myristate-13-acetate-induced protein 1, PMA-Induced Protein 1, Protein Noxa

Entrez Gene IDs

5366 (Human); 58801 (Mouse)

Gene Symbol

PMAIP1

UniProt

Q13794 (Human)

Additional Noxa Products

Product Documents for Noxa Antibody (114C307.1) - Azide Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Noxa Antibody (114C307.1) - Azide Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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