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Nse2 Antibody (OTI6A6)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-02956

Novus Biologicals, part of Bio-Techne
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NBP2-02956
Conjugate
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Key Product Details

Species Reactivity

Validated:

Human

Applications

Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # OTI6A6

Concentration

0.52 mg/ml

Product Summary for Nse2 Antibody (OTI6A6)

Immunogen

Full length human recombinant protein of human NSMCE2 (NP_775956) produced in E.coli.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Theoretical MW

27.8 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Nse2 Antibody (OTI6A6)

Western Blot: Nse2 Antibody (OTI6A6) [NBP2-02956]

Western Blot: Nse2 Antibody (OTI6A6) [NBP2-02956]

Western Blot: Nse2 Antibody (6A6) [NBP2-02956] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Nse2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-Nse2.
Immunocytochemistry/Immunofluorescence: Nse2 Antibody (6A6) [NBP2-02956] Staining of COS7 cells transiently transfected by pCMV6-ENTRY Nse2.
Flow Cytometry: Nse2 Antibody (OTI6A6) [NBP2-02956]

Flow Cytometry: Nse2 Antibody (OTI6A6) [NBP2-02956]

Flow Cytometry: Nse2 Antibody (6A6) [NBP2-02956] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-Nse2 antibody, and then analyzed by flow cytometry.

Applications for Nse2 Antibody (OTI6A6)

Application
Recommended Usage

Flow Cytometry

1:100

Immunocytochemistry/ Immunofluorescence

1:100

Western Blot

1:2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.3), 1.0% BSA and 50% Glycerol

Preservative

0.02% Sodium Azide

Concentration

0.52 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Nse2

E3 SUMO-protein ligase component of the SMC5-SMC6 complex, a complex involved in DNA double-strand breaks byhomologous recombination. The complex may promote sister chromatid homologous recombination by recruiting theSMC1-SMC3 cohesin complex to double-strand breaks. Acts as a E3 ligase mediating SUMO attachment to various proteinssuch as SMC6L1 and TRAX, and maybe the cohesin components RAD21 and STAG2. SUMO protein-ligase activity is requiredfor the prevention of DNA damage-induced apoptosis by facilitating DNA repair

Alternate Names

C8orf36, E3 SUMO-protein ligase NSE2, EC 6.3.2, EC 6.3.2.-, FLJ32440, hMMS21, methyl methanesulfonate sensitivity gene 21, MMS21 homolog, MMS21chromosome 8 open reading frame 36, Non-SMC element 2 homolog, non-SMC element 2 homolog (MMS21, S. cerevisiae), non-SMC element 2, MMS21 homolog (S. cerevisiae), Non-structural maintenance of chromosomes element 2 homolog, NSE2

Entrez Gene IDs

286053 (Human)

Gene Symbol

NSMCE2

Additional Nse2 Products

Product Documents for Nse2 Antibody (OTI6A6)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Nse2 Antibody (OTI6A6)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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