Nse2 Antibody (OTI6A6) - Azide and BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-73070
![Flow Cytometry: Nse2 Antibody (OTI6A6) - Azide and BSA Free [NBP2-73070]](https://resources.bio-techne.com/images/products/Nse2-Antibody-OTI6A6-Azide-and-BSA-Free-Flow-Cytometry-NBP2-73070-img0002.jpg "Flow Cytometry: Nse2 Antibody (OTI6A6) - Azide and BSA Free [NBP2-73070]")
![Western Blot: Nse2 Antibody (OTI6A6)Azide and BSA Free [NBP2-73070]](https://resources.bio-techne.com/images/products/Nse2-Antibody-OTI6A6-Azide-and-BSA-Free-Western-Blot-NBP2-73070-img0001.jpg "Western Blot: Nse2 Antibody (OTI6A6)Azide and BSA Free [NBP2-73070]")
Conjugate
Catalog #
Key Product Details
Species Reactivity
Validated:
Human
Applications
CyTOF-ready, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2A Clone # OTI6A6
Format
Azide and BSA Free
Concentration
LYOPH mg/ml
Product Summary for Nse2 Antibody (OTI6A6) - Azide and BSA Free
Immunogen
Full length human recombinant protein of human NSMCE2 (NP_775956) produced in E.coli.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2A
Theoretical MW
27.8 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for Nse2 Antibody (OTI6A6) - Azide and BSA Free
Western Blot: Nse2 Antibody (OTI6A6)Azide and BSA Free [NBP2-73070]
Western Blot: Nse2 Antibody (OTI6A6) - Azide and BSA Free [NBP2-73070] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Nse2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-Nse2.![Immunocytochemistry/ Immunofluorescence: Nse2 Antibody (OTI6A6) - Azide and BSA Free [NBP2-73070]](https://resources.bio-techne.com/images/products/Nse2-Antibody-OTI6A6-Azide-and-BSA-Free-Immunocytochemistry-Immunofluorescence-NBP2-73070-img0004.jpg "Immunocytochemistry/ Immunofluorescence: Nse2 Antibody (OTI6A6) - Azide and BSA Free [NBP2-73070]")
Immunocytochemistry/ Immunofluorescence: Nse2 Antibody (OTI6A6) - Azide and BSA Free [NBP2-73070]
Immunocytochemistry/Immunofluorescence: Nse2 Antibody (OTI6A6) - Azide and BSA Free [NBP2-73070] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY Nse2.![Flow Cytometry: Nse2 Antibody (OTI6A6) - Azide and BSA Free [NBP2-73070]](https://resources.bio-techne.com/images/products/Nse2-Antibody-OTI6A6-Azide-and-BSA-Free-Flow-Cytometry-NBP2-73070-img0003.jpg "Flow Cytometry: Nse2 Antibody (OTI6A6) - Azide and BSA Free [NBP2-73070]")
Flow Cytometry: Nse2 Antibody (OTI6A6) - Azide and BSA Free [NBP2-73070]
Flow Cytometry: Nse2 Antibody (OTI6A6) - Azide and BSA Free [NBP2-73070] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostaining by anti-Nse2 antibody, and then analyzed by flow cytometry.Applications for Nse2 Antibody (OTI6A6) - Azide and BSA Free
Application
Recommended Usage
Flow Cytometry
1:100
Immunocytochemistry/ Immunofluorescence
1:100
Western Blot
1:2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Reconstitution
we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.
Formulation
Lyophilized from PBS (pH 7.3) with 8% Trehalose
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
LYOPH mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: Nse2
Alternate Names
C8orf36, E3 SUMO-protein ligase NSE2, EC 6.3.2, EC 6.3.2.-, FLJ32440, hMMS21, methyl methanesulfonate sensitivity gene 21, MMS21 homolog, MMS21chromosome 8 open reading frame 36, Non-SMC element 2 homolog, non-SMC element 2 homolog (MMS21, S. cerevisiae), non-SMC element 2, MMS21 homolog (S. cerevisiae), Non-structural maintenance of chromosomes element 2 homolog, NSE2
Gene Symbol
NSMCE2
Additional Nse2 Products
Product Documents for Nse2 Antibody (OTI6A6) - Azide and BSA Free
Product Specific Notices for Nse2 Antibody (OTI6A6) - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Loading...
Loading...
Loading...
Loading...
Loading...
Loading...