NTH1 Antibody (22H10)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-26456
Recombinant monoclonal antibody expressed in HEK293F cells
Key Product Details
Species Reactivity
Validated:
Human
Applications
ELISA, Flow Cytometry, Immunocytochemistry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # 22H10
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Summary for NTH1 Antibody (22H10)
Immunogen
A synthesized peptide derived from Human NTH1 [UniProt P78549]
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for NTH1 Antibody (22H10)
Immunohistochemistry: NTH1 Antibody (22H10) [NBP3-26456] -
Immunohistochemistry: NTH1 Antibody (22H10) [NBP3-26456] - Image of NTH1 Antibody (22H10) diluted at 1:100 and staining in paraffin-embedded human testis tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.
Western Blot: NTH1 Antibody (22H10) [NBP3-26456] -
Western Blot: NTH1 Antibody (22H10) [NBP3-26456] - Positive Western Blot detected in: HepG2 whole cell lysate, HEK293 whole cell lysate, PC-3 whole cell lysate.All lanes: NTH1 Antibody at 1: 1000
Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution.
Predicted band size: 35, 34, 33 kDa
Observed band size: 35 kDa
Immunocytochemistry/Immunofluorescence: NTH1 Antibody (22H10) [NBP3-26456] -
Immunocytochemistry/Immunofluorescence: NTH1 Antibody (22H10) [NBP3-26456] - Staining of PC-3 cell with NTH1 Antibody (22H10) at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 604-conjugated Goat Anti-Rabbit IgG (H+L).Applications for NTH1 Antibody (22H10)
Application
Recommended Usage
Flow Cytometry
1:50-1:200
Immunocytochemistry
/Immunofluorescence 1:50-1:200
Immunocytochemistry/ Immunofluorescence
1:50-1:200
Western Blot
1:500-1:2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS, pH 7.4, 150mM NaCl and 50% glycerol
Preservative
0.02% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20 to -70C. Avoid freeze-thaw cycles.
Background: NTH1
Long Name
Nth Endonuclease III-like 1
Alternate Names
NTHL1, OCTS3
Gene Symbol
NTHL1
Additional NTH1 Products
Product Documents for NTH1 Antibody (22H10)
Product Specific Notices for NTH1 Antibody (22H10)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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