Occludin Antibody (CL1555)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-52971

Novus Biologicals, part of Bio-Techne
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NBP2-52971
NBP2-52971-25ul
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Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Human

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # CL1555

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all Occludin Antibodies »

Product Specifications

Immunogen

This antibody was developed against a recombinant protein corresponding to amino acids: DKEHIYDEQPPNVEEWVKNVSAGTQDVPSPPSDYVERVDSPMAYSSNGKVNDKRFYPESSYKSTPVPEVVQELPLTSPVDDFRQPRYSSGGNFETPSKRAPAKGRAGRSKRTEQDHYETDYTTGGESCDELEED

Epitope

TSPVDDFRQPRYSSG

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (85%), Rat (83%).

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Scientific Data Images for Occludin Antibody (CL1555)

Immunohistochemistry-Paraffin: Occludin Antibody (CL1555) [NBP2-52971]

Immunohistochemistry-Paraffin: Occludin Antibody (CL1555) [NBP2-52971]

Immunohistochemistry-Paraffin: Occludin Antibody (CL1555) [NBP2-52971] - Analysis in human stomach and skeletal muscle tissues using NBP2-52971 antibody. Corresponding OCLN RNA-seq data are presented for the same tissues.
Western Blot: Occludin Antibody (CL1555) [NBP2-52971]

Western Blot: Occludin Antibody (CL1555) [NBP2-52971]

Western Blot: Occludin Antibody (CL1555) [NBP2-52971] - Lane 1: Marker [kDa], Lane 2: Negative control (vector only transfected HEK293T lysate), Lane 3: OCLN Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells)
Immunocytochemistry/ Immunofluorescence: Occludin Antibody (CL1555) [NBP2-52971]

Immunocytochemistry/ Immunofluorescence: Occludin Antibody (CL1555) [NBP2-52971]

Immunocytochemistry/Immunofluorescence: Occludin Antibody (CL1555) [NBP2-52971] - staining of CACO-2 cells using the Anti-OCLN monoclonal antibody, showing specific staining in cell junctions in green. Microtubule- and nuclear probes are visualized in red and blue, respectively (where available).
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Applications for Occludin Antibody (CL1555)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

2-10 ug/ml

Immunohistochemistry

1:1000 - 1:2500

Immunohistochemistry-Paraffin

1:1000 - 1:2500

Western Blot

1 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation/Permeabilization: PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Occludin

The Occludin gene encodes an integral membrane protein which is located at tight junctions. This protein may be involved in the formation and maintenance of the tight junction.

Alternate Names

BLCPMG, OCLN

Gene Symbol

OCLN

Additional Occludin Products

Product Documents for Occludin Antibody (CL1555)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Occludin Antibody (CL1555)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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