Occludin Antibody (CL1555) - Azide and BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-43902
![Occludin Antibody (CL1555) - Azide and BSA Free Immunohistochemistry-Paraffin: Occludin Antibody [NBP3-43902]](https://resources.bio-techne.com/images/products/nbp3-43902_mouse-occludin-mab-cl1555-azide-and-bsa-free-282202512145368.jpg "Immunohistochemistry-Paraffin: Occludin Antibody [NBP3-43902]")
![Occludin Antibody (CL1555) - Azide and BSA Free Immunohistochemistry-Paraffin: Occludin Antibody [NBP3-43902]](https://resources.bio-techne.com/images/products/nbp3-43902_mouse-occludin-mab-cl1555-azide-and-bsa-free-282202512324827.jpg "Immunohistochemistry-Paraffin: Occludin Antibody [NBP3-43902]")
![Occludin Antibody (CL1555) - Azide and BSA Free Western Blot: Occludin Antibody [NBP3-43902]](https://resources.bio-techne.com/images/products/nbp3-43902_mouse-occludin-mab-cl1555-azide-and-bsa-free-282202512173173.jpg "Western Blot: Occludin Antibody [NBP3-43902]")
Conjugate
Catalog #
Forumulation
Catalog #
Key Product Details
Validated by
Orthogonal Validation
Species Reactivity
Human
Applications
Immunocytochemistry/ Immunofluorescence, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2A Clone # CL1555
Format
Azide and BSA Free
Concentration
LYOPH mg/ml
Product Specifications
Immunogen
This antibody was generated using a recombinant protein sequence of Q16625, with the exact immunogen sequence remaining proprietary.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2A
Scientific Data Images for Occludin Antibody (CL1555) - Azide and BSA Free
Western Blot: Occludin Antibody [NBP3-43902]
Lane 1: Marker [kDa]Lane 2: Human cell line CACO-2
Lane 3: Human cell line U-87
![Occludin Antibody (CL1555) - Azide and BSA Free Immunocytochemistry/ Immunofluorescence: Occludin Antibody [NBP3-43902]](https://resources.bio-techne.com/images/products/nbp3-43902_mouse-occludin-mab-cl1555-azide-and-bsa-free-28220251210428.jpg "Immunocytochemistry/ Immunofluorescence: Occludin Antibody [NBP3-43902]")
Immunocytochemistry/ Immunofluorescence: Occludin Antibody [NBP3-43902]
Staining of CACO-2 cells using the Anti-Occludin monoclonal antibody) .![Occludin Antibody (CL1555) - Azide and BSA Free Immunohistochemistry-Paraffin: Occludin Antibody [NBP3-43902]](https://resources.bio-techne.com/images/products/nbp3-43902_mouse-occludin-mab-cl1555-azide-and-bsa-free-28220251239445.jpg "Immunohistochemistry-Paraffin: Occludin Antibody [NBP3-43902]")
![Occludin Antibody (CL1555) - Azide and BSA Free Immunohistochemistry-Paraffin: Occludin Antibody [NBP3-43902]](https://resources.bio-techne.com/images/products/nbp3-43902_mouse-occludin-mab-cl1555-azide-and-bsa-free-282202512303845.jpg "Immunohistochemistry-Paraffin: Occludin Antibody [NBP3-43902]")
![Occludin Antibody (CL1555) - Azide and BSA Free Immunohistochemistry-Paraffin: Occludin Antibody [NBP3-43902]](https://resources.bio-techne.com/images/products/nbp3-43902_mouse-occludin-mab-cl1555-azide-and-bsa-free-282202512405566.jpg "Immunohistochemistry-Paraffin: Occludin Antibody [NBP3-43902]")
![Occludin Antibody (CL1555) - Azide and BSA Free Immunohistochemistry-Paraffin: Occludin Antibody [NBP3-43902]](https://resources.bio-techne.com/images/products/nbp3-43902_mouse-occludin-mab-cl1555-azide-and-bsa-free-28220251375663.jpg "Immunohistochemistry-Paraffin: Occludin Antibody [NBP3-43902]")
![Occludin Antibody (CL1555) - Azide and BSA Free Immunohistochemistry-Paraffin: Occludin Antibody [NBP3-43902]](https://resources.bio-techne.com/images/products/nbp3-43902_mouse-occludin-mab-cl1555-azide-and-bsa-free-282202513121898.jpg "Immunohistochemistry-Paraffin: Occludin Antibody [NBP3-43902]")
![Occludin Antibody (CL1555) - Azide and BSA Free Immunohistochemistry-Paraffin: Occludin Antibody [NBP3-43902]](https://resources.bio-techne.com/images/products/nbp3-43902_mouse-occludin-mab-cl1555-azide-and-bsa-free-282202513245411.jpg "Immunohistochemistry-Paraffin: Occludin Antibody [NBP3-43902]")
Applications for Occludin Antibody (CL1555) - Azide and BSA Free
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
2-10 ug/ml
Immunohistochemistry-Paraffin
1:1000 - 1:2500
Western Blot
1 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/ Immunofluorescence/IF Fixation/Permeabilization: PFA/Triton X-100.
Formulation, Preparation, and Storage
Purification
Protein A purified
Reconstitution
Centrifuge the vial of lyophilized antibody at 12,000 x g for 20 seconds. Reconstitute by adding sterile, distilled water to achieve a final antibody concentration of 1mg/ml.
Formulation
Lyophilized from a 0.2 um filtered solution in PBS with Trehalose
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
LYOPH mg/ml
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: Occludin
Alternate Names
BLCPMG, OCLN
Gene Symbol
OCLN
Additional Occludin Products
Product Documents for Occludin Antibody (CL1555) - Azide and BSA Free
Product Specific Notices for Occludin Antibody (CL1555) - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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