Occludin Antibody (OCLN/2181)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-07137
![Western Blot: Occludin Antibody (OCLN/2181) [NBP3-07137]](https://resources.bio-techne.com/images/products/Occludin-Antibody-OCLN-2181-Western-Blot-NBP3-07137-img0003.jpg "Western Blot: Occludin Antibody (OCLN/2181) [NBP3-07137]")
Conjugate
Catalog #
Forumulation
Catalog #
Key Product Details
Species Reactivity
Human
Applications
Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 kappa Clone # OCLN/2181
Concentration
0.2 mg/ml
Product Specifications
Immunogen
Recombinant fragment of human Occludin protein (around aa 282-415) (exact sequence is proprietary) (Uniprot: Q16625)
Localization
Cell Surface and Cytoplasmic
Marker
Marker of Early Blood Brain Barrier Damage
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1 kappa
Description
200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP3-08879)
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
Scientific Data Images for Occludin Antibody (OCLN/2181)
Western Blot: Occludin Antibody (OCLN/2181) [NBP3-07137]
Western Blot: Occludin Antibody (OCLN/2181) [NBP3-07137] - Western Blot Analysis of MCF-7 cell lysate with Occludin Mouse Monoclonal Antibody (OCLN/2181).![Immunohistochemistry-Paraffin: Occludin Antibody (OCLN/2181) [NBP3-07137]](https://resources.bio-techne.com/images/products/Occludin-Antibody-OCLN-2181-Immunohistochemistry-Paraffin-NBP3-07137-img0002.jpg "Immunohistochemistry-Paraffin: Occludin Antibody (OCLN/2181) [NBP3-07137]")
Immunohistochemistry-Paraffin: Occludin Antibody (OCLN/2181) [NBP3-07137]
Immunohistochemistry-Paraffin: Occludin Antibody (OCLN/2181) [NBP3-07137] - Formalin-fixed, paraffin-embedded human thyroid carcinoma stained with Occludin Mouse Monoclonal Antibody (OCLN/2181).![Immunohistochemistry-Paraffin: Occludin Antibody (OCLN/2181) [NBP3-07137]](https://resources.bio-techne.com/images/products/Occludin-Antibody-OCLN-2181-Immunohistochemistry-Paraffin-NBP3-07137-img0001.jpg "Immunohistochemistry-Paraffin: Occludin Antibody (OCLN/2181) [NBP3-07137]")
Immunohistochemistry-Paraffin: Occludin Antibody (OCLN/2181) [NBP3-07137]
Immunohistochemistry-Paraffin: Occludin Antibody (OCLN/2181) [NBP3-07137] - Formalin-fixed, paraffin-embedded human Renal Cell Carcinoma stained with Occludin Mouse Monoclonal Antibody (OCLN/2181).Applications for Occludin Antibody (OCLN/2181)
Application
Recommended Usage
Immunohistochemistry-Paraffin
1-2 ug/ml
Western Blot
1-2 ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.
Optimal dilution for a specific application should be determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
10 mM PBS with 0.05% BSA
Preservative
0.05% Sodium Azide
Concentration
0.2 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C.
Background: Occludin
Additional Occludin Products
Product Documents for Occludin Antibody (OCLN/2181)
Product Specific Notices for Occludin Antibody (OCLN/2181)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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