Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # OCLN/2181

Format

Azide and BSA Free

Concentration

1 mg/ml

View all Occludin Antibodies »

Product Specifications

Immunogen

Recombinant fragment of human Occludin protein (around aa 282-415) (exact sequence is proprietary) (Uniprot: Q16625)

Localization

Cell Surface and Cytoplasmic

Marker

Marker of Early Blood Brain Barrier Damage

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Description

1.0 mg/ml of antibody purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS WITHOUT BSA & azide. Also available at 200 ug/ml WITH BSA & azide (NBP3-07137).

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.

Scientific Data Images for Occludin Antibody (OCLN/2181) - Azide and BSA Free

Western Blot: Occludin Antibody (OCLN/2181)Azide and BSA Free [NBP3-08879]

Western Blot: Occludin Antibody (OCLN/2181)Azide and BSA Free [NBP3-08879]

Western Blot: Occludin Antibody (OCLN/2181) - Azide and BSA Free [NBP3-08879] - Western Blot Analysis of MCF-7 cell lysate with Occludin Mouse Monoclonal Antibody (OCLN/2181).
Immunohistochemistry-Paraffin: Occludin Antibody (OCLN/2181) - Azide and BSA Free [NBP3-08879]

Immunohistochemistry-Paraffin: Occludin Antibody (OCLN/2181) - Azide and BSA Free [NBP3-08879]

Immunohistochemistry-Paraffin: Occludin Antibody (OCLN/2181) - Azide and BSA Free [NBP3-08879] - Formalin-fixed, paraffin-embedded human thyroid carcinoma stained with Occludin Mouse Monoclonal Antibody (OCLN/2181).
Immunohistochemistry-Paraffin: Occludin Antibody (OCLN/2181) - Azide and BSA Free [NBP3-08879]

Immunohistochemistry-Paraffin: Occludin Antibody (OCLN/2181) - Azide and BSA Free [NBP3-08879]

Immunohistochemistry-Paraffin: Occludin Antibody (OCLN/2181) - Azide and BSA Free [NBP3-08879] - Formalin-fixed, paraffin-embedded human Renal Cell Carcinoma stained with Occludin Mouse Monoclonal Antibody (OCLN/2181).

Applications for Occludin Antibody (OCLN/2181) - Azide and BSA Free

Application
Recommended Usage

Immunohistochemistry-Paraffin

1-2 ug/ml

Western Blot

1-2 ug/ml
Application Notes
Immunohistochemistry Formalin-fixed: 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10 mM PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20 to -80C. Avoid freeze-thaw cycles.

Background: Occludin

The Occludin gene encodes an integral membrane protein which is located at tight junctions. This protein may be involved in the formation and maintenance of the tight junction.

Alternate Names

BLCPMG, OCLN

Gene Symbol

OCLN

Additional Occludin Products

Product Documents for Occludin Antibody (OCLN/2181) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for Occludin Antibody (OCLN/2181) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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