Ornithine Carbamoyltransferase Antibody (CL4045)
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-59038
![Immunohistochemistry-Paraffin: Ornithine Carbamoyltransferase Antibody (CL4045) [NBP2-59038]](https://resources.bio-techne.com/images/products/Ornithine-Carbamoyltransferase-Antibody-CL4045-Immunohistochemistry-Paraffin-NBP2-59038-img0007.jpg "Immunohistochemistry-Paraffin: Ornithine Carbamoyltransferase Antibody (CL4045) [NBP2-59038]")
![Immunohistochemistry-Paraffin: Ornithine Carbamoyltransferase Antibody (CL4045) [NBP2-59038]](https://resources.bio-techne.com/images/products/Ornithine-Carbamoyltransferase-Antibody-CL4045-Immunohistochemistry-Paraffin-NBP2-59038-img0012.jpg "Immunohistochemistry-Paraffin: Ornithine Carbamoyltransferase Antibody (CL4045) [NBP2-59038]")
Key Product Details
Validated by
Orthogonal Validation
Species Reactivity
Validated:
Human, Cynomolgus Monkey
Cited:
Primate - Macaca fascicularis (Crab-eating Monkey or Cynomolgus Macaque)
Predicted:
Mouse (93%), Rat (92%). Backed by our 100% Guarantee.
Applications
Validated:
Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Cited:
Immunohistochemistry-Paraffin
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2A Clone # CL4045
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
This antibody was developed against a recombinant protein corresponding to the following amino acid sequence:ILADYLTLQEHYSSLKGLTLSWIGDGNNILHSIMMSAAKFGMHLQAATPKGYEPDASVTKLAEQYAKENGTKLLLTNDPLEAAHGGNVLITDTWISMGQEEEKKKRLQAFQGYQVTMKTAKVAASDWT
Epitope
GYEPDASVTKLAEQY
Reactivity Notes
Use in Cynomolgus Monkey reported in scientific literature (PMID:35211641). Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2A
Scientific Data Images for Ornithine Carbamoyltransferase Antibody (CL4045)
![Western Blot: Ornithine Carbamoyltransferase Antibody (CL4045) [NBP2-59038]](https://resources.bio-techne.com/images/products/Ornithine-Carbamoyltransferase-Antibody-CL4045-Western-Blot-NBP2-59038-img0006.jpg "Western Blot: Ornithine Carbamoyltransferase Antibody (CL4045) [NBP2-59038]")
Western Blot: Ornithine Carbamoyltransferase Antibody (CL4045) [NBP2-59038]
Western Blot: Ornithine Carbamoyltransferase Antibody (CL4045) [NBP2-59038] - Analysis in human liver tissue.![Immunocytochemistry/ Immunofluorescence: Ornithine Carbamoyltransferase Antibody (CL4045) [NBP2-59038]](https://resources.bio-techne.com/images/products/Ornithine-Carbamoyltransferase-Antibody-CL4045-Immunocytochemistry-Immunofluorescence-NBP2-59038-img0013.jpg "Immunocytochemistry/ Immunofluorescence: Ornithine Carbamoyltransferase Antibody (CL4045) [NBP2-59038]")
Immunocytochemistry/ Immunofluorescence: Ornithine Carbamoyltransferase Antibody (CL4045) [NBP2-59038]
Immunocytochemistry/Immunofluorescence: Ornithine Carbamoyltransferase Antibody (CL4045) [NBP2-59038] - Staining of RH-30 cells using the Anti-OTC monoclonal antibody, showing specific staining in mitochondria in green. Microtubule- and nuclear probes are visualized in red and blue, respectively (where available).![Immunohistochemistry-Paraffin: Ornithine Carbamoyltransferase Antibody (CL4045) [NBP2-59038]](https://resources.bio-techne.com/images/products/Ornithine-Carbamoyltransferase-Antibody-CL4045-Immunohistochemistry-Paraffin-NBP2-59038-img0008.jpg "Immunohistochemistry-Paraffin: Ornithine Carbamoyltransferase Antibody (CL4045) [NBP2-59038]")
![Immunohistochemistry-Paraffin: Ornithine Carbamoyltransferase Antibody (CL4045) [NBP2-59038]](https://resources.bio-techne.com/images/products/Ornithine-Carbamoyltransferase-Antibody-CL4045-Immunohistochemistry-Paraffin-NBP2-59038-img0009.jpg "Immunohistochemistry-Paraffin: Ornithine Carbamoyltransferase Antibody (CL4045) [NBP2-59038]")
![Immunohistochemistry-Paraffin: Ornithine Carbamoyltransferase Antibody (CL4045) [NBP2-59038]](https://resources.bio-techne.com/images/products/Ornithine-Carbamoyltransferase-Antibody-CL4045-Immunohistochemistry-Paraffin-NBP2-59038-img0010.jpg "Immunohistochemistry-Paraffin: Ornithine Carbamoyltransferase Antibody (CL4045) [NBP2-59038]")
Applications for Ornithine Carbamoyltransferase Antibody (CL4045)
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
2-10 ug/ml
Immunohistochemistry
1:10000 - 1:20000
Immunohistochemistry-Paraffin
1:10000 - 1:20000
Western Blot
1 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. For ICC/IF, PFA/Triton X-100 is recommended for fixation/permeabilization.
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: Ornithine Carbamoyltransferase/OTC
Alternate Names
OCTD, Ornithine Transcarbamylase, OTC, OTCase
Gene Symbol
OTC
Additional Ornithine Carbamoyltransferase/OTC Products
Product Documents for Ornithine Carbamoyltransferase Antibody (CL4045)
Product Specific Notices for Ornithine Carbamoyltransferase Antibody (CL4045)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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