Ornithine Carbamoyltransferase Antibody (CL4045) - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-44105

Novus Biologicals, part of Bio-Techne
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NBP3-44105
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NBP2-59038
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Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Human, Cynomolgus Monkey

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # CL4045

Format

Azide and BSA Free

Concentration

LYOPH mg/ml

View all Ornithine Carbamoyltransferase/OTC Antibodies »

Product Specifications

Immunogen

This antibody was generated using a recombinant protein sequence of P00480, with the exact immunogen sequence remaining proprietary.

Reactivity Notes

Use in Cynomolgus Monkey reported in scientific literature (PMID:35211641). Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Scientific Data Images for Ornithine Carbamoyltransferase Antibody (CL4045) - Azide and BSA Free

Ornithine Carbamoyltransferase Antibody (CL4045) - Azide and BSA Free Immunohistochemistry-Paraffin: Ornithine Carbamoyltransferase Antibody [NBP3-44105]

Immunohistochemistry-Paraffin: Ornithine Carbamoyltransferase Antibody [NBP3-44105]

Analysis in human liver and tonsil tissues using NBP3-44105 antibody. Corresponding Ornithine Carbamoyltransferase RNA-seq data are presented for the same tissues.
Ornithine Carbamoyltransferase Antibody (CL4045) - Azide and BSA Free Immunohistochemistry-Paraffin: Ornithine Carbamoyltransferase Antibody [NBP3-44105]

Immunohistochemistry-Paraffin: Ornithine Carbamoyltransferase Antibody [NBP3-44105]

Staining of human duodenum shows strong granular cytoplasmic positivity in glandular cells.
Ornithine Carbamoyltransferase Antibody (CL4045) - Azide and BSA Free Immunohistochemistry-Paraffin: Ornithine Carbamoyltransferase Antibody [NBP3-44105]

Immunohistochemistry-Paraffin: Ornithine Carbamoyltransferase Antibody [NBP3-44105]

Staining of human liver shows strong granular cytoplasmic positivity in hepatocytes.
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Applications for Ornithine Carbamoyltransferase Antibody (CL4045) - Azide and BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

2-10 ug/ml

Immunohistochemistry-Paraffin

1:10000 - 1:20000

Western Blot

1 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. For Immunocytochemistry/ Immunofluorescence/IF, PFA/Triton X-100 is recommended for fixation/permeabilization.

Formulation, Preparation, and Storage

Purification

Protein A purified

Reconstitution

Centrifuge the vial of lyophilized antibody at 12,000 x g for 20 seconds. Reconstitute by adding sterile, distilled water to achieve a final antibody concentration of 1mg/ml.

Formulation

Lyophilized in 5% Trehalose.

Format

Azide and BSA Free

Preservative

0.02% Sodium Azide

Concentration

LYOPH mg/ml

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Ornithine Carbamoyltransferase/OTC

This nuclear gene encodes a mitochondrial matrix enzyme. Missense, nonsense, and frameshift mutations in this enzyme lead to ornithine transcarbamylase deficiency, which causes hyperammonemia. Since the gene for this enzyme maps close to that for Duchenne muscular dystrophy, it may play a role in that disease also. (provided by RefSeq)

Alternate Names

OCTD, Ornithine Transcarbamylase, OTC, OTCase

Gene Symbol

OTC

Additional Ornithine Carbamoyltransferase/OTC Products

Product Documents for Ornithine Carbamoyltransferase Antibody (CL4045) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Ornithine Carbamoyltransferase Antibody (CL4045) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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