Otubain-1 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-83670

Novus Biologicals, part of Bio-Techne
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NBP1-83670
NBP1-83670-25ul
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Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Knockdown Validated, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all Otubain-1 Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: AAEEPQQQKQEPLGSDSEGVNCLAYDEAIMAQQDRIQQEIAVQNPLVSERLELSVLYKEYAEDDNIYQQKIKDLHKKYSYIRKTRPD

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Otubain-1 Antibody

Western Blot: Otubain-1 Antibody [NBP1-83670]

Western Blot: Otubain-1 Antibody [NBP1-83670]

Western Blot: Otubain-1 Antibody [NBP1-83670] - Analysis in MCF-7 cells transfected with control siRNA, target specific siRNA probe #1 and #2. Remaining relative intensity is presented. Loading control: Anti-PPIB.
Immunohistochemistry-Paraffin: Otubain-1 Antibody [NBP1-83670]

Immunohistochemistry-Paraffin: Otubain-1 Antibody [NBP1-83670]

Immunohistochemistry-Paraffin: Otubain-1 Antibody [NBP1-83670] - Staining of human Fallopian tube shows moderate cytoplasmic positivity in glandular cells.
Western Blot: Otubain-1 Antibody [NBP1-83670]

Western Blot: Otubain-1 Antibody [NBP1-83670]

Western Blot: Otubain-1 Antibody [NBP1-83670] - Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.

Applications for Otubain-1 Antibody

Application
Recommended Usage

Immunohistochemistry

1:50 - 1:200

Immunohistochemistry-Paraffin

1:50 - 1:200

Western Blot

0.04 - 0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Otubain-1

The product of this gene is a member of the OTU (ovarian tumor) superfamily of predicted cysteine proteases. The encoded protein is a highly specific ubiquitin iso-peptidase, and cleaves ubiquitin from branched poly-ubiquitin chains but not from ubiquitinated substrates. It interacts with another ubiquitin protease and an E3 ubiquitin ligase that inhibits cytokine gene transcription in the immune system. It is proposed to function in specific ubiquitin-dependent pathways, possibly by providing an editing function for polyubiquitin chain growth. Alternative splicing results in multiple transcript variants.

Long Name

OTU Domain, Ubiquitin Aldehyde Binding 1

Alternate Names

OTB1, OTU1, OTUB1, Otubain1

Gene Symbol

OTUB1

Additional Otubain-1 Products

Product Documents for Otubain-1 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Otubain-1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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