p27/Kip1 Antibody (KIP1/769)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-44495

Novus Biologicals, part of Bio-Techne
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NBP2-44495-0.1mg
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat, Monkey

Applications

Flow (Intracellular), Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # KIP1/769

Concentration

0.2 mg/ml

View all p27/Kip1 Antibodies »

Product Summary for p27/Kip1 Antibody (KIP1/769)

Immunogen

Recombinant full-length human p27/Kip1 protein (Uniprot: P46527)

Localization

Nuclear

Specificity

This monoclonal antibody recognizes a 27kDa protein, identified as the p27Kip1, a cell cycle regulatory mitotic inhibitor. It is highly specific and shows no cross-reaction with other related mitotic inhibitors. p27Kip1 functions as a negative regulator of G1 progression and has been proposed to function as a possible mediator of TGF- induced G1 arrest. p27Kip1 is a candidate tumor suppressor gene. This monoclonal antibody is excellent for staining of formalin-fixed tissues.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Description

200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP2-47769)

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.

Scientific Data Images for p27/Kip1 Antibody (KIP1/769)

Immunocytochemistry/ Immunofluorescence: p27/Kip1 Antibody (KIP1/769) [NBP2-44495]

Immunocytochemistry/ Immunofluorescence: p27/Kip1 Antibody (KIP1/769) [NBP2-44495]

Immunocytochemistry/Immunofluorescence: p27/Kip1 Antibody (KIP1/769) [NBP2-44495] - Analysis of PFA-fixed MCF-7 cells labeling p27 with p27/Kip1 Antibody (KIP1/769) followed by Goat anti-Mouse IgG-CF488 (Green). Membrane is stained with Phalloidin-CF640.
Immunohistochemistry-Paraffin: p27/Kip1 Antibody (KIP1/769) [NBP2-44495]

Immunohistochemistry-Paraffin: p27/Kip1 Antibody (KIP1/769) [NBP2-44495]

Immunohistochemistry-Paraffin: p27/Kip1 Antibody (KIP1/769) [NBP2-44495] - Rat Colon stained with p27 Monoclonal Antibody (KIP1/769)
Flow Cytometry: p27/Kip1 Antibody (KIP1/769) [NBP2-44495]

Flow Cytometry: p27/Kip1 Antibody (KIP1/769) [NBP2-44495]

Flow Cytometry: p27/Kip1 Antibody (KIP1/769) [NBP2-44495] - Human p27 on Jurkat Cells. Black: Cells alone; Grey: Isotype Control; Green: AF488-labeled p27 Monoclonal Antibody (KIP1/769).
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Applications for p27/Kip1 Antibody (KIP1/769)

Application
Recommended Usage

Flow Cytometry

1-2 ug/million cells

Immunocytochemistry/ Immunofluorescence

1-2 ug/ml

Immunohistochemistry-Paraffin

0.25-0.5 ug/ml

Western Blot

1-2 ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 0.25-0.5ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10 mM PBS with 0.05% BSA

Preservative

0.05% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C.

Background: p27/Kip1

p27(Kip1) is a cyclin-dependent kinase inhibitor involved in G1 arrest. p27(Kip1) binds to and inhibits cyclinE-Cdk2 complex, cyclinA-CDK2 and cyclinD1-CDK4 (1). p27(Kip1) is regulated by phosphorylation on serine 10 (S10) and threonine 187 (T187). Phosphorylation by CDK2 on T187 results in ubiquitylation and degradation of p27(Kip1) (2); while phosphorylation by hKIS on S10 signals the nuclear export to the cytoplasm (3).

Long Name

p27 Cyclin Dependent Kinase 4 Inhibitor 1B

Alternate Names

CDKN1B, Kip1

Gene Symbol

CDKN1B

UniProt

P46527

Additional p27/Kip1 Products

Product Documents for p27/Kip1 Antibody (KIP1/769)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for p27/Kip1 Antibody (KIP1/769)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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