p27/Kip1 Antibody (KIP1/769) - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-47769

Novus Biologicals, part of Bio-Techne
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NBP2-47769-0.1mg
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Key Product Details

Species Reactivity

Human, Mouse, Rat, Monkey

Applications

CyTOF-ready, Flow (Intracellular), Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # KIP1/769

Format

Azide and BSA Free

Concentration

1.0 mg/ml

View all p27/Kip1 Antibodies »

Product Specifications

Immunogen

Recombinant full-length human p27/Kip1 protein (Uniprot: P46527)

Localization

Nuclear

Specificity

This monoclonal antibody recognizes a 27kDa protein, identified as the p27Kip1, a cell cycle regulatory mitotic inhibitor. It is highly specific and shows no cross-reaction with other related mitotic inhibitors. p27Kip1 functions as a negative regulator of G1 progression and has been proposed to function as a possible mediator of TGF- induced G1 arrest. p27Kip1 is a candidate tumor suppressor gene. This monoclonal antibody is excellent for staining of formalin-fixed tissues.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Description

1.0 mg/ml of antibody purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS WITHOUT BSA & azide. Also available at 200 ug/ml WITH BSA & azide (NBP2-44495).

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.

Scientific Data Images for p27/Kip1 Antibody (KIP1/769) - Azide and BSA Free

Immunocytochemistry/ Immunofluorescence: p27/Kip1 Antibody (KIP1/769) - Azide and BSA Free [NBP2-47769]

Immunocytochemistry/ Immunofluorescence: p27/Kip1 Antibody (KIP1/769) - Azide and BSA Free [NBP2-47769]

Immunocytochemistry/Immunofluorescence: p27/Kip1 Antibody (KIP1/769) - Azide and BSA Free [NBP2-47769] - Analysis of PFA-fixed MCF-7 cells labeling p27 with p27/Kip1 Antibody (KIP1/769) followed by Goat anti-Mouse IgG-CF488 (Green). Membrane is stained with Phalloidin-CF640.
Immunohistochemistry-Paraffin: p27/Kip1 Antibody (KIP1/769) - Azide and BSA Free [NBP2-47769]

Immunohistochemistry-Paraffin: p27/Kip1 Antibody (KIP1/769) - Azide and BSA Free [NBP2-47769]

Immunohistochemistry-Paraffin: p27/Kip1 Antibody (KIP1/769) - Azide and BSA Free [NBP2-47769] - Rat Colon stained with p27 Monoclonal Antibody (KIP1/769)
Flow (Intracellular): p27/Kip1 Antibody (KIP1/769) - Azide and BSA Free [NBP2-47769]

Flow (Intracellular): p27/Kip1 Antibody (KIP1/769) - Azide and BSA Free [NBP2-47769]

Flow (Intracellular): p27/Kip1 Antibody (KIP1/769) - Azide and BSA Free [NBP2-47769] - An intracellular stain was performed on Jurkat cells with p27/Kip1 Antibody (KIP1/769) NBP2-47769PE and a matched isotype control. Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Phycoerythrin. Image using the Azide and BSA Free form of this antibody.
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Applications for p27/Kip1 Antibody (KIP1/769) - Azide and BSA Free

Application
Recommended Usage

Flow Cytometry

0.5 - 1 ug/million cells in 0.1 ml

Immunofluorescence

0.5 - 1.0 ug/ml

Immunohistochemistry-Paraffin

0.25 - 0.5 ug/ml

Western Blot

0.5 - 1.0 ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 0.25-0.5ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10 mM PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20 to -80C. Avoid freeze-thaw cycles.

Background: p27/Kip1

p27(Kip1) is a cyclin-dependent kinase inhibitor involved in G1 arrest. p27(Kip1) binds to and inhibits cyclinE-Cdk2 complex, cyclinA-CDK2 and cyclinD1-CDK4 (1). p27(Kip1) is regulated by phosphorylation on serine 10 (S10) and threonine 187 (T187). Phosphorylation by CDK2 on T187 results in ubiquitylation and degradation of p27(Kip1) (2); while phosphorylation by hKIS on S10 signals the nuclear export to the cytoplasm (3).

Long Name

p27 Cyclin Dependent Kinase 4 Inhibitor 1B

Alternate Names

CDKN1B, Kip1

Gene Symbol

CDKN1B

Additional p27/Kip1 Products

Product Documents for p27/Kip1 Antibody (KIP1/769) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for p27/Kip1 Antibody (KIP1/769) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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