p27/Kip1 Antibody (KIP1/769) - IHC-Prediluted

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-48399

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat, Monkey

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # KIP1/769

Format

IHC-Prediluted

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all p27/Kip1 Antibodies »

Product Summary for p27/Kip1 Antibody (KIP1/769) - IHC-Prediluted

Immunogen

Recombinant full-length human p27/Kip1 protein (Uniprot: P46527)

Localization

Nuclear

Specificity

This monoclonal antibody recognizes a 27kDa protein, identified as the p27Kip1, a cell cycle regulatory mitotic inhibitor. It is highly specific and shows no cross-reaction with other related mitotic inhibitors. p27Kip1 functions as a negative regulator of G1 progression and has been proposed to function as a possible mediator of TGF- induced G1 arrest. p27Kip1 is a candidate tumor suppressor gene. This monoclonal antibody is excellent for staining of formalin-fixed tissues.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Description

The prediluted antibody does not require any mixing, dilution, reconstitution, or titration; the antibody is ready-to-use and optimized for staining.

Scientific Data Images for p27/Kip1 Antibody (KIP1/769) - IHC-Prediluted

Immunohistochemistry-Paraffin: p27/Kip1 Antibody (KIP1/769) - IHC-Prediluted [NBP2-48399]

Immunohistochemistry-Paraffin: p27/Kip1 Antibody (KIP1/769) - IHC-Prediluted [NBP2-48399]

Immunohistochemistry-Paraffin: p27/Kip1 Antibody (KIP1/769) - IHC-Prediluted [NBP2-48399] - Rat Colon stained with p27 Monoclonal Antibody (KIP1/769)
Immunohistochemistry-Paraffin: p27/Kip1 Antibody (KIP1/769) - IHC-Prediluted [NBP2-48399]

Immunohistochemistry-Paraffin: p27/Kip1 Antibody (KIP1/769) - IHC-Prediluted [NBP2-48399]

Immunohistochemistry-Paraffin: p27/Kip1 Antibody (KIP1/769) - IHC-Prediluted [NBP2-48399] - Human Cervical cancer stained with p27 Monoclonal Antibody (KIP1/769)
Immunohistochemistry-Paraffin: p27/Kip1 Antibody (KIP1/769) - IHC-Prediluted [NBP2-48399]

Immunohistochemistry-Paraffin: p27/Kip1 Antibody (KIP1/769) - IHC-Prediluted [NBP2-48399]

Immunohistochemistry-Paraffin: p27/Kip1 Antibody (KIP1/769) - IHC-Prediluted [NBP2-48399] - Human Colon Carcinoma stained with p27 Monoclonal Antibody (KIP1/769)
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Applications for p27/Kip1 Antibody (KIP1/769) - IHC-Prediluted

Application
Recommended Usage

Immunohistochemistry

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry-Paraffin

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Immunohistochemistry (Formalin-fixed): 0.25-0.5ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10 mM PBS with 0.05% BSA

Format

IHC-Prediluted

Preservative

0.05% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C.

Background: p27/Kip1

p27(Kip1) is a cyclin-dependent kinase inhibitor involved in G1 arrest. p27(Kip1) binds to and inhibits cyclinE-Cdk2 complex, cyclinA-CDK2 and cyclinD1-CDK4 (1). p27(Kip1) is regulated by phosphorylation on serine 10 (S10) and threonine 187 (T187). Phosphorylation by CDK2 on T187 results in ubiquitylation and degradation of p27(Kip1) (2); while phosphorylation by hKIS on S10 signals the nuclear export to the cytoplasm (3).

Long Name

p27 Cyclin Dependent Kinase 4 Inhibitor 1B

Alternate Names

CDKN1B, Kip1

Gene Symbol

CDKN1B

Additional p27/Kip1 Products

Product Documents for p27/Kip1 Antibody (KIP1/769) - IHC-Prediluted

Product Datasheets

Certificate of Analysis

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Product Specific Notices for p27/Kip1 Antibody (KIP1/769) - IHC-Prediluted

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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