p40/deltaNp63 Antibody (P40/8225R)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-20376
Recombinant Monoclonal Antibody
Conjugate
Catalog #
Forumulation
Catalog #
Key Product Details
Species Reactivity
Validated:
Human
Applications
Immunohistochemistry-Paraffin
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Kappa Clone # P40/8225R
Concentration
0.2 mg/ml
Product Summary for p40/deltaNp63 Antibody (P40/8225R)
Immunogen
Recombinant fragment (around aa1-200) of human p40/deltaNp63 protein (exact sequence is proprietary)
Localization
Nucleus.
Specificity
The p40 (clone P40/8225R) antibody recognizes exclusively delta-Np63 but not TAp63 p40 is a squamous cell carcinoma specific antibody It reacts with the vast majority of cases of squamous cell carcinomas of various origins, but not with adenocarcinomas It is particularly useful in differentiating lung squamous cell carcinoma from lung poorly differentiated adenocarcinoma p40 antibody can also be used as an alternative basal cell/myoepithelial cell marker, which has similar sensitivity and specificity as that of p63 antibody Therefore, p40 antibody may also be used as an alternative immunohistochemical marker for determining prostate adenocarcinoma vs benign prostate glands and for determining breast intraductal carcinoma vs invasive breast ductal carcinoma
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG Kappa
Description
Positive Controls: HeLa cells. Human prostate or lung tissue (IHC).
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C. Non-hazardous. No MSDS required.
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C. Non-hazardous. No MSDS required.
Scientific Data Images for p40/deltaNp63 Antibody (P40/8225R)
p40/deltaNp63 Antibody (P40/8225R)
Formalin-fixed, paraffin-embedded human prostate carcinoma stained with p40/deltaNp63 antibody (P40/8225R). HIER: Tris/EDTA, pH9.0, 45min. 2: HRP-polymer, 30min. DAB, 5min.
p40/deltaNp63 Antibody (P40/8225R)
Formalin-fixed, paraffin-embedded human lung carcinoma stained with p40/deltaNp63 antibody (P40/8225R). HIER: Tris/EDTA, pH9.0, 45min. 2: HRP-polymer, 30min. DAB, 5min.Applications for p40/deltaNp63 Antibody (P40/8225R)
Application
Recommended Usage
Immunohistochemistry-Paraffin
1-2 ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes. at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.
Optimal dilution for a specific application should be determined.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
10mM PBS with 0.05% BSA
Preservative
0.05% Sodium Azide
Concentration
0.2 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C. Do not freeze.
Background: p40/deltaNp63
Alternate Names
AIS, amplified in squamous cell carcinoma, B(p51A), B(p51B), Chronic ulcerative stomatitis protein, EEC3, Keratinocyte transcription factor KET, KET, LMS, OFC8, p53CP, SHFM4, TP53CP, TP53L, TP63, TP73L, Transformation-related protein 63, tumor protein p53-competing protein
Gene Symbol
TP63
Additional p40/deltaNp63 Products
Product Documents for p40/deltaNp63 Antibody (P40/8225R)
Product Specific Notices for p40/deltaNp63 Antibody (P40/8225R)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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