p53 Antibody (TRP/816)

Knockout/Knockdown

Unconjugated

Monoclonal Mouse IgG2a Kappa Clone # TRP/816

0.2 mg/ml

Recombinant human full-length p53 protein (Uniprot: P04637)

Nuclear

The specificity of this monoclonal antibody to its intended target was validated by HuProtTM Array, containing more than 19,000, full-length human proteins. Recognizes a 53kDa protein, which is identified as p53 suppressor gene product. It reacts with the mutant as well as the wild form of p53 under denaturing and non-denaturing conditions. p53 is a tumor suppressor gene expressed in a wide variety of tissue types and is involved in regulating cell growth, replication, and apoptosis. It binds to MDM2, SV40 T antigen and human papilloma virus E6 protein. Positive nuclear staining with p53 antibody has been reported to be a negative prognostic factor in breast carcinoma, lung carcinoma, colorectal, and urothelial carcinoma. Anti-p53 positivity has also been used to differentiate uterine serous carcinoma from endometrioid carcinoma as well as to detect intratubular germ cell neoplasia. Mutations involving p53 are found in a wide variety of malignant tumors, including breast, ovarian, bladder, colon, lung, and melanoma.

Monoclonal

Mouse

IgG2a Kappa

53 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Human Chromosome Location:17p13.1


200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP2-59628)

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.

Protein A or G purified

10 mM PBS with 0.05% BSA

0.05% Sodium Azide

0.2 mg/ml

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Store at 4C.