Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Human

Applications

CyTOF-ready, Immunohistochemistry, Immunohistochemistry-Paraffin, Knockout Validated, Protein Array, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2a Kappa Clone # TRP/816

Format

Azide and BSA Free

Concentration

1.0 mg/ml

View all p53 Antibodies »

Product Specifications

Immunogen

Recombinant human full-length p53 protein (Uniprot: P04637)

Localization

Nuclear

Specificity

The specificity of this monoclonal antibody to its intended target was validated by HuProtTM Array, containing more than 19,000, full-length human proteins. Recognizes a 53kDa protein, which is identified as p53 suppressor gene product. It reacts with the mutant as well as the wild form of p53 under denaturing and non-denaturing conditions. p53 is a tumor suppressor gene expressed in a wide variety of tissue types and is involved in regulating cell growth, replication, and apoptosis. It binds to MDM2, SV40 T antigen and human papilloma virus E6 protein. Positive nuclear staining with p53 antibody has been reported to be a negative prognostic factor in breast carcinoma, lung carcinoma, colorectal, and urothelial carcinoma. Anti-p53 positivity has also been used to differentiate uterine serous carcinoma from endometrioid carcinoma as well as to detect intratubular germ cell neoplasia. Mutations involving p53 are found in a wide variety of malignant tumors, including breast, ovarian, bladder, colon, lung, and melanoma.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Theoretical MW

53 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

Human Chromosome Location:17p13.1


1.0 mg/ml of antibody purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS WITHOUT BSA & azide. Also available at 200 ug/ml WITH BSA & azide (NBP2-59626).

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.

Scientific Data Images for p53 Antibody (TRP/816) - Azide and BSA Free

Knockout Validated: p53 Antibody (TRP/816) - Azide and BSA Free [NBP2-59628]

Knockout Validated: p53 Antibody (TRP/816) - Azide and BSA Free [NBP2-59628]

Knockout Validated: p53 Antibody (TRP/816) - Azide and BSA Free [NBP2-59628] - Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and p53 knockout (KO) HeLa cell line. PVDF membrane was probed with 0.5 ug/ml of Mouse Anti-Human HSP27 Monoclonal Antibody (Catalog # NBP2-59628) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (catalog number HAF018). Specific band was detected for p53 at approximately 53 kDa (as indicated) in the parental HeLa cell line, but is not detectable in the knockout HeLa cell line. This experiment was conducted under reducing conditions.
Western Blot: p53 Antibody (TRP/816)Azide and BSA Free [NBP2-59628]

Western Blot: p53 Antibody (TRP/816)Azide and BSA Free [NBP2-59628]

Western Blot: p53 Antibody (TRP/816) - Azide and BSA Free [NBP2-59628] - Western Blot Analysis of human HeLa cell lysate using p53 Antibody (TRP/816).
Immunohistochemistry-Paraffin: p53 Antibody (TRP/816) - Azide and BSA Free [NBP2-59628]

Immunohistochemistry-Paraffin: p53 Antibody (TRP/816) - Azide and BSA Free [NBP2-59628]

Immunohistochemistry-Paraffin: p53 Antibody (TRP/816) - Azide and BSA Free [NBP2-59628] - Formalin-fixed, paraffin-embedded human Bladder Carcinoma stained with p53 Mouse Monoclonal Antibody (TRP/816).
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Applications for p53 Antibody (TRP/816) - Azide and BSA Free

Application
Recommended Usage

Immunohistochemistry-Paraffin

0.5-1 ug/ml

Western Blot

0.5-1 ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10 mM PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20 to -80C. Avoid freeze-thaw cycles.

Background: p53

p53 is a stress-regulated transcription factor that was first identified as an SV40 large T antigen-binding protein. It plays a major role in the cellular response to DNA damage and other genomic aberrations. The activation of p53 can lead to either cell cycle arrest and DNA repair, or apoptosis. p53 is phosphorylated at multiple sites in vivo and by several different protein kinases in vitro. Mutation of p53 is the most common genetic change so far identified in a number of major carcinomas. This antibody can be used for the specific detection of human p53 that is synthesized in the presence of p53 from other species.

Alternate Names

BCC7, LFS1, TP53, TRP53

Gene Symbol

TP53

Additional p53 Products

Product Documents for p53 Antibody (TRP/816) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for p53 Antibody (TRP/816) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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