PAK1 Antibody (4F10)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-15141

Recombinant monoclonal antibody expressed in HEK293F cells
Novus Biologicals, part of Bio-Techne
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NBP3-15141
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Key Product Details

Species Reactivity

Human, Mouse

Applications

ELISA, Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 4F10

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all PAK1 Antibodies »

Product Specifications

Immunogen

A synthesized peptide derived from human PAK1. UniProt ID (Q13153).

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for PAK1 Antibody (4F10)

PAK1 Antibody (4F10)

Western Blot: PAK1 Antibody (4F10) [NBP3-15141]

Western Blot: PAK1 Antibody (4F10) [NBP3-15141] - Positive WB detected in: MCF7 whole cell lysate, JK whole cell lysate, K562 whole cell lysate, A549 whole cell lysate, ntera2 whole cell lysate, Mouse Heart tissue lysate, Mouse Skeletal Muscle tissue lysate, Mouse Brain tissue lysate, Rat Brain tissue lysate
All lanes: PAK1 antibody at 1:1000
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 61 kDa
Observed band size: 61 kDa
PAK1 Antibody (4F10)

Immunocytochemistry/Immunofluorescence: PAK1 Antibody (4F10) [NBP3-15141]

Immunocytochemistry/Immunofluorescence: PAK1 Antibody (4F10) [NBP3-15141] - Staining of Hela cell with NBP3-15141 at 1:30, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L).

Applications for PAK1 Antibody (4F10)

Application
Recommended Usage

Western Blot

1:500-1:5000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, pH 7.4, 150mM NaCl, and 50% glycerol

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20 to -70C. Avoid freeze-thaw cycles.

Background: PAK1

p21-activated kinase 1 (PAK1) is a member of a family of serine/threonine protein kinase defined by their interaction with the small GTPases, Cdc42 and Rac (1-2). The PAK proteins can be essentially divided into two categories, groups I (PAK1, PAK2 and PAK3) and group II (PAK4, PAK5 and PAK6), based on their structures (3). PAK1 participates in regulating the actin cytoskeleton, focal adhesion contacts, cell motility, apoptosis and transcription. It also promotes the disassembly of stress fibers (4-5). PAK1 also binds PIX (PAK-binding protein), COOL and adapter protein Nck (5).

Long Name

p21/Cdc42/Rac1-activated Kinase 1

Alternate Names

alpha-PAK

Gene Symbol

PAK1

Additional PAK1 Products

Product Documents for PAK1 Antibody (4F10)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for PAK1 Antibody (4F10)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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