PAK2 Antibody (1E1)

Novus Biologicals, part of Bio-Techne | Catalog # H00005062-M01

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Human

Applications

ELISA, Proximity Ligation Assay, Sandwich ELISA, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 1E1

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all PAK2 Antibodies »

Product Specifications

Immunogen

PAK2 (NP_002568, 131 a.a. ~ 230 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. DSNTVKQKYLSFTPPEKDGFPSGTPALNAKGTEAPAVVTEEEDDDEETAPPVIAPRPDHTKSIYTRSVIDPVPAPVGDSHVDGAAKSLDKQKKKTKMTDE

Specificity

p21 (CDKN1A)-activated kinase 2

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for PAK2 Antibody (1E1)

Western Blot: PAK2 Antibody (1E1) [H00005062-M01]

Western Blot: PAK2 Antibody (1E1) [H00005062-M01]

Western Blot: PAK2 Antibody (1E1) [H00005062-M01] - PAK2 monoclonal antibody (M01), clone 1E1. Analysis of PAK2 expression in human liver.
Proximity Ligation Assay: PAK2 Antibody (1E1) [H00005062-M01]

Proximity Ligation Assay: PAK2 Antibody (1E1) [H00005062-M01]

Proximity Ligation Assay: PAK2 Antibody (1E1) [H00005062-M01] - Analysis of protein-protein interactions between CASP3 and PAK2. HeLa cells were stained with anti-CASP3 rabbit purified polyclonal 1:1200 and anti-PAK2 mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).
Sandwich ELISA: PAK2 Antibody (1E1) [H00005062-M01] - Detection limit for recombinant GST tagged PAK2 is 0.1 ng/ml as a capture antibody.

Applications for PAK2 Antibody (1E1)

Application
Recommended Usage

Western Blot

1:500
Application Notes
Antibody reactivity against Recombinant Protein with GST tag on ELISA and WB. GST tag alone is used as a negative control.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: PAK2

The p21 activated kinases (PAK) are critical effectors that link Rho GTPases to cytoskeleton reorganization and nuclear signaling. The PAK proteins are a family of serine/threonine kinases that serve as targets for the small GTP binding proteins, CDC42 and RAC1, and have been implicated in a wide range of biological activities. The protein encoded by this gene is activated by proteolytic cleavage during caspase-mediated apoptosis, and may play a role in regulating the apoptotic events in the dying cell.

Long Name

p21-activated Kinase 2

Alternate Names

gamma-PAK, PAK65, S6/H4 Kinase

Entrez Gene IDs

5062 (Human)

Gene Symbol

PAK2

OMIM

605022 (Human)

UniProt

NP_002568 (Human)

Additional PAK2 Products

Product Documents for PAK2 Antibody (1E1)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for PAK2 Antibody (1E1)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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