Panendothelial Cell Antigen Antibody (MECA-32) - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NB100-77668

Novus Biologicals, part of Bio-Techne
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NB100-77668SS
NB100-77668
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Key Product Details

Species Reactivity

Validated:

Human, Mouse

Cited:

Human, Mouse

Applications

Validated:

CyTOF-ready, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot

Cited:

IF/IHC, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Rat IgG2a Kappa Clone # MECA-32

Format

BSA Free

Concentration

1.0 mg/ml

View all Panendothelial Cell Antigen Antibodies »

Product Specifications

Immunogen

Mouse lymph node stromal cells.

Specificity

Pan endothelial cells except for CNS & muscle capillaries.

Clonality

Monoclonal

Host

Rat

Isotype

IgG2a Kappa

Scientific Data Images for Panendothelial Cell Antigen Antibody (MECA-32) - BSA Free

Western Blot: Panendothelial Cell Antigen Antibody (MECA-32)BSA Free [NB100-77668]

Western Blot: Panendothelial Cell Antigen Antibody (MECA-32)BSA Free [NB100-77668]

Western Blot: Panendothelial Cell Antigen Antibody (MECA-32) [NB100-77668] - Total protein from human Colon, Thyroid and Stomach was separated on a 12% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-Panendothelial Cell Antigen (NB100-77668) in blocking buffer and detected with an anti-rat HRP secondary antibody using NovaLume chemiluminescence detection reagent (NPB2-61915).
Immunohistochemistry-Paraffin: Panendothelial Cell Antigen Antibody (MECA-32) - BSA Free [NB100-77668]

Immunohistochemistry-Paraffin: Panendothelial Cell Antigen Antibody (MECA-32) - BSA Free [NB100-77668]

Immunohistochemistry-Paraffin: Panendothelial Cell Antigen Antibody (MECA-32) [NB100-77668] - Panendothelial Cell Antigen (MECA-32) was detected in immersion fixed paraffin-embedded sections of human spleen. Antibody at 1:500 for 1 hour at room temperature followed by incubation with the Anti-Rat IgG VisUCyte(TM) HRP Polymer Antibody (Catalog # VC005). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to the endothelial cells.
Flow Cytometry: Panendothelial Cell Antigen Antibody (MECA-32) - BSA Free [NB100-77668]

Flow Cytometry: Panendothelial Cell Antigen Antibody (MECA-32) - BSA Free [NB100-77668]

Flow Cytometry: Panendothelial Cell Antigen Antibody (MECA-32) [NB100-77668] - Analysis using the PerCP conjugate of NB100-77668. Staining of bEND.3 cells with MECA-32 AF488.
View 1 more images

Applications for Panendothelial Cell Antigen Antibody (MECA-32) - BSA Free

Application
Recommended Usage

Flow Cytometry

<= 0.25 ug/10^6 cells in 100 ul

Immunocytochemistry/ Immunofluorescence

1:10-1:500

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Frozen

1:10-1:500

Immunohistochemistry-Paraffin

1:200 - 1:500. Use reported in scientific literature (PMID 26586691)

Immunoprecipitation

1:10-1:500

Western Blot

2 ug/ml
Application Notes
There have been mixed results for use in Immunohistochemisty on paraffin-embedded sections.

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Panendothelial Cell Antigen

MECA-32 is a single-pass type II membrane homodimer glycoprotein (50-55 kDa) primarily expressed on most endothelial cells in embryonic and adult mouse tissues, except in the brain, skeletal, and cardiac muscle, where expression is more constrained. In the brain, it is only expressed in the circumventricular organs and the neurohemal tissues. In skeletal and cardiac muscle, MECA-32 is confined in small arterioles and venules. In cases such as cardiac transplantation or inflammation, MECA-32 expression can be induced on some negative-cardiac endothelial cells. During embryonic development, MECA-32 antigen is expressed on the brain vasculature up to the first 16 days of gestation and disappears thereafter. The cessation of MECA-32 antigen expression in the CNS may be associated with the establishment of the blood-brain barrier, which begins on day 16 of gestation. In the adult mouse, inflammation in the CNS can lead to re-expression of the panendothelial cell antigen.

Alternate Names

FELS, Fenestrated endothelial-linked structure protein, gp68, MECA32, MECA-32, Panendothelial Cell Antigen, plasmalemma vesicle associated protein, Plasmalemma Vesicle Protein 1, PV-1 protein

Entrez Gene IDs

84094 (Mouse)

Gene Symbol

PLVAP

UniProt

Q91VC4 (Mouse)

Additional Panendothelial Cell Antigen Products

Product Documents for Panendothelial Cell Antigen Antibody (MECA-32) - BSA Free

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Panendothelial Cell Antigen Antibody (MECA-32) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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