Panendothelial Cell Antigen Antibody (MECA-32) [Janelia Fluor® 549]

Novus Biologicals, part of Bio-Techne | Catalog # NB100-77668JF549

Novus Biologicals, part of Bio-Techne
Catalog #
Availability
Size / Price
Qty
NB100-77668JF549
Print Quote
Bulk Order
100% Guarantee

Key Product Details

Species Reactivity

Human, Mouse

Applications

CyTOF-ready, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot

Label

Janelia Fluor 549

Antibody Source

Monoclonal Rat IgG2a Kappa Clone # MECA-32

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all Panendothelial Cell Antigen Antibodies »

Product Specifications

Immunogen

Mouse lymph node stromal cells.

Specificity

Pan endothelial cells except for CNS & muscle capillaries.

Clonality

Monoclonal

Host

Rat

Isotype

IgG2a Kappa

Applications for Panendothelial Cell Antigen Antibody (MECA-32) [Janelia Fluor® 549]

Application
Recommended Usage

CyTOF-ready

Optimal dilutions of this antibody should be experimentally determined.

Flow Cytometry

Optimal dilutions of this antibody should be experimentally determined.

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry-Frozen

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry-Paraffin

Optimal dilutions of this antibody should be experimentally determined.

Immunoprecipitation

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

50mM Sodium Borate

Preservative

0.05% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C in the dark.

Background: Panendothelial Cell Antigen

MECA-32 is a single-pass type II membrane homodimer glycoprotein (50-55 kDa) primarily expressed on most endothelial cells in embryonic and adult mouse tissues, except in the brain, skeletal, and cardiac muscle, where expression is more constrained. In the brain, it is only expressed in the circumventricular organs and the neurohemal tissues. In skeletal and cardiac muscle, MECA-32 is confined in small arterioles and venules. In cases such as cardiac transplantation or inflammation, MECA-32 expression can be induced on some negative-cardiac endothelial cells. During embryonic development, MECA-32 antigen is expressed on the brain vasculature up to the first 16 days of gestation and disappears thereafter. The cessation of MECA-32 antigen expression in the CNS may be associated with the establishment of the blood-brain barrier, which begins on day 16 of gestation. In the adult mouse, inflammation in the CNS can lead to re-expression of the panendothelial cell antigen.

Alternate Names

FELS, Fenestrated endothelial-linked structure protein, gp68, MECA32, MECA-32, Panendothelial Cell Antigen, plasmalemma vesicle associated protein, Plasmalemma Vesicle Protein 1, PV-1 protein

Gene Symbol

Plvap

Additional Panendothelial Cell Antigen Products

Product Documents

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for Panendothelial Cell Antigen Antibody (MECA-32) [Janelia Fluor® 549]

Sold under license from the Howard Hughes Medical Institute, Janelia Research Campus.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Privacy and Cookie Policy
Site Map
© Copyright 2024 Bio-Techne. All Rights Reserved.