PAR1/Thrombin Receptor Antibody (N2-11) [DyLight 405]

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-71770V

Submit a review

Product Datasheet / COA / SDS

View Available Conjugates View Available Formulations

Conjugate

Catalog #

Unconjugated

NBP1-71770

Alexa Fluor 350

NBP1-71770AF350

Alexa Fluor 405

NBP1-71770AF405

Alexa Fluor 488

NBP1-71770AF488

Alexa Fluor 532

NBP1-71770AF532

Alexa Fluor 594

NBP1-71770AF594

Alexa Fluor 647

NBP1-71770AF647

Alexa Fluor 700

NBP1-71770AF700

Alexa Fluor 750

NBP1-71770AF750

Biotin

NBP1-71770B

CoraFluor 1

NBP1-71770CL1

DyLight 350

NBP1-71770UV

DyLight 488

NBP1-71770G

DyLight 550

NBP1-71770R

DyLight 594

NBP1-71770DL594

DyLight 650

NBP1-71770C

DyLight 680

NBP1-71770FR

DyLight 755

NBP1-71770IR

FITC

NBP1-71770F

HRP

NBP1-71770H

Janelia Fluor 525

NBP1-71770JF525

Janelia Fluor 549

NBP1-71770JF549

Janelia Fluor 585

NBP1-71770JF585

Janelia Fluor 635

NBP1-71770JF635

Janelia Fluor 646

NBP1-71770JF646

Janelia Fluor 669

NBP1-71770JF669

mFluor Violet 450 SE

NBP1-71770MFV450

mFluor Violet 500 SE

NBP1-71770MFV500

mFluor Violet 610 SE

NBP1-71770MFV610

Forumulation

Catalog #

Azide and BSA Free

NBP2-80904

Product Summary
Applications
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Species Reactivity

Validated:

Human, Mouse

Applications

ELISA, Immunocytochemistry/ Immunofluorescence, Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Western Blot

Label

DyLight 405 (Excitation = 400 nm, Emission = 420 nm)

Antibody Source

Monoclonal Mouse IgG₁ Clone # N2-11

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all PAR1 Antibodies »

Product Summary for PAR1/Thrombin Receptor Antibody (N2-11) [DyLight 405]

Immunogen

The oligopeptide CNATLDPRSFLL from human Thrombin Receptor. [Swiss-Prot# P25116]

Localization

Cell membrane; Multi-pass membrane protein.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG₁

Applications for PAR1/Thrombin Receptor Antibody (N2-11) [DyLight 405]

Application

Recommended Usage

ELISA

Optimal dilutions of this antibody should be experimentally determined.

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry-Frozen

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry-Paraffin

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.

Application Notes

Optimal dilution of this antibody should be experimentally determined.

Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

50mM Sodium Borate

Preservative

0.05% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C in the dark.

Background: PAR1

Thrombin, the main effector protease of coagulation cascade, is a most potent platelet activator and its actions on platelets are mediated by Thrombin Receptors (also called platelet protease-activated receptors, PARs), which are GPCR members of 7-transmembrane domain receptor superfamily. PARs subtypes includes: PAR-1, PAR-2, PAR-3, and PAR-4, and in addition to platelets, they are also located in smooth muscle cells, endothelial cells, and fibroblasts. PAR1 or thrombin receptor (F2R) is a prototypical GPCR activated by thrombin's lowest concentration compared to other PARs and after cleavage of the extreme N-terminal peptide, the exposed tethered ligand of PAR1 activates itself for participation in diverse physiological processes including coagulation, inflammation, and vascular homeostasis. PAR1 knockout in mouse leads to death of pups in midgestation due to impaired vascular development as well as severe bleeding, whereas endothelial cell-specific reintroduction of PAR1 can rescue the phenotype. PAR1 has emerged as a target for anti-platelet therapies which are useful for ACS (acute coronary syndromes), stable coronary disease, ischemic atherothrombotic stroke, and peripheral arterial disease.

Long Name

Protease-Activated Receptor 1

Alternate Names

Cf2r, F2R, HTR, ThrR, TRGPC

Gene Symbol

F2R

Additional PAR1 Products

Product Documents for PAR1/Thrombin Receptor Antibody (N2-11) [DyLight 405]

Product Datasheets

Download Product Datasheets

COA

SDS

Product Specific Notices for PAR1/Thrombin Receptor Antibody (N2-11) [DyLight 405]

DyLight (R) is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations
Reviews