PAR2 Antibody (JE35-05)

Western Blot: PAR2 Antibody (JE35-05) [NBP3-32715]

Western Blot: PAR2 Antibody (JE35-05) [NBP3-32715] - Western blot analysis of PAR2 on different lysates with Rabbit anti-PAR2 antibody (NBP3-32715) at 1/1,000 dilution.

Lane 1: HCT 116 cell lysate (15 ug/Lane)
Lane 2: HepG2 cell lysate (15 ug/Lane)
Lane 3: PANC-1 cell lysate (15 ug/Lane)
Lane 4: PC-3M cell lysate (15 ug/Lane)
Lane 5: AGS cell lysate (15 ug/Lane)
Lane 6: SGC-7901 cell lysate (15 ug/Lane)
Lane 7: Human kidney tissue lysate (30 ug/Lane)
Lane 8: Human stomach tissue lysate (30 ug/Lane)
Lane 9: Mouse large intestine tissue lysate (30 ug/Lane)

Predicted band size: 44 kDa
Observed band size: 55 kDa (Glycosylation)

Exposure time: 1 minute 40 seconds;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32715) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.

Flow Cytometry: PAR2 Antibody (JE35-05) [NBP3-32715]

Flow Cytometry: PAR2 Antibody (JE35-05) [NBP3-32715] - Flow cytometric analysis of HepG2 cells labeling PAR2.

Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32715, 1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).