Park7/DJ-1 Antibody (4H4)

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-92715

Novus Biologicals, part of Bio-Techne

Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Validated:

Human, Bovine, Mouse (Negative), Rat (Negative)

Cited:

Human

Applications

Validated:

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Knockout Validated, Simple Western, Western Blot

Cited:

Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 4H4

Concentration

1 mg/ml

View all Park7/DJ-1 Antibodies »

Product Specifications

Immunogen

Full length recombinant human Park7(DJ-1) expressed in and purified from E. coli. [UniProt# Q99497]

Reactivity Notes

Not reactive with rodent.

Localization

Cytoplasm. Nucleus. Mitochondrion. Note: Under normal conditions, located predominantly in the cytoplasm and, to a lesser extent, in the nucleus and mitochondrion. Translocates to the mitochondrion and subsequently to the nucleus in response to oxidative stress and exerts an increased cytoprotective effect against oxidative damage. Detected in tau inclusions in brains from neurodegenerative disease patients.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

21 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Park7/DJ-1 Antibody (4H4)

Knockout Validated: Park7/DJ-1 Antibody (4H4) [NBP1-92715]

Knockout Validated: Park7/DJ-1 Antibody (4H4) [NBP1-92715]

Knockout Validated: Park7/DJ-1 Antibody (4H4) [NBP1-92715] - Western blot shows lysates of 293T human embryonic kidney parental cell line and PARK7 knockout (KO) 293T cell line. PVDF membrane was probed with Mouse Anti-Human PARK7 Monoclonal Antibody (Catalog # NBP1-92715) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog #HAF008). Specific band was detected for PARK7 at approximately 23 kDa (as indicated) in the parental 293T cell line, but is not detectable in the knockout 293T cell line. This experiment was conducted under reducing conditions.
Western Blot: Park7/DJ-1 Antibody (4H4) [NBP1-92715]

Western Blot: Park7/DJ-1 Antibody (4H4) [NBP1-92715]

Western Blot: Park7/DJ-1 Antibody (4H4) [NBP1-92715] - Analysis of whole brain and cell lysates using mouse mAb against DJ1/Park7, NBP1-92715, dilution 1:5,000 in green. [1] protein standard, [2] rat brain, [3] mouse brain, [4] NIH-3T3, [5] HeLa, [6] HEK293, and [7] C6 cells. The DJ1 antibody detects protein with apparent molecular weight of 21kDa but only in human cell lines, since it does not recognize the rat or mouse DJ1 protein. The blot was simultaneously probed with chicken pAb to vimentin, dilution 1;5,000 in red, revealing a single band at about 50kDa present in all lanes, though at much lower levels in the tissue lysates.
Immunocytochemistry/ Immunofluorescence: Park7/DJ-1 Antibody (4H4) [NBP1-92715]

Immunocytochemistry/ Immunofluorescence: Park7/DJ-1 Antibody (4H4) [NBP1-92715]

Immunocytochemistry/Immunofluorescence: Park7(DJ-1) Antibody (4H4) [NBP1-92715] - HeLa cells stained with NBP1-92715 (green), chicken antibody to Vimentin (NB300-223, red) and DNA (blue). NBP1-92715 reveals strong cytoplasmic staining for Park7(DJ-1).
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Applications for Park7/DJ-1 Antibody (4H4)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:5000

Immunohistochemistry

1:5000

Simple Western

1:500

Western Blot

1:5000
Application Notes
In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

50% PBS, 50% glycerol

Preservative

0.035% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Park7/DJ-1

Park7(DJ-1) (Parkinson disease protein 7/ oncogene DJ1, also known as CAP1) was initially discovered as an oncogene promoting Ras-dependent transformation and later it was identified as being responsible for an autosomal recessive early-onset form of Parkinson's disease type 7 (PARK7). Member of peptidase C56 family, Park7(DJ-1) exists as homodimer located predominantly in the cytoplasm to a lesser extent in nucleus as well as mitochondrion, and besides being an essential part of a ternary complex containing PARK7, EFCAB6/DJBP and AR, it interacts with OTUD7B, BBS1, HIPK1, CLCF1, MTERF, EFCAB6/DJBP and PIAS2. Park7(DJ-1) is an evolutionary conserved ubiquitously expressed multifunctional protein involved in several biological processes including transcriptional regulation, oxidative stress, carcinogenesis, cell growth and transformation, AR-dependent transcription regulation, apoptosis, inflammation, redox-sensitive chaperon activities, protein degradation, fertilization and regulation of mitochondrial morphology /function as well as for autophagy of dysfunctional mitochondria.

Long Name

Parkinson Disease 7

Alternate Names

DJ-1, DJ1

Entrez Gene IDs

11315 (Human)

Gene Symbol

PARK7

UniProt

Q99497

Additional Park7/DJ-1 Products

Product Documents for Park7/DJ-1 Antibody (4H4)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Park7/DJ-1 Antibody (4H4)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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