Park7/DJ-1 Antibody (4H4) [DyLight 488]
Novus Biologicals, part of Bio-Techne | Catalog # NBP1-92715G
Conjugate
Catalog #
Key Product Details
Species Reactivity
Validated:
Human, Bovine, Mouse (Negative), Rat (Negative)
Applications
Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Knockout Validated, Simple Western, Western Blot
Label
DyLight 488 (Excitation = 493 nm, Emission = 518 nm)
Antibody Source
Monoclonal Mouse IgG1 Clone # 4H4
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Summary for Park7/DJ-1 Antibody (4H4) [DyLight 488]
Immunogen
Full length recombinant human Park7(DJ-1) expressed in and purified from E. coli. [UniProt# Q99497]
Reactivity Notes
Not reactive with rodent.
Localization
Cytoplasm. Nucleus. Mitochondrion. Note: Under normal conditions, located predominantly in the cytoplasm and, to a lesser extent, in the nucleus and mitochondrion. Translocates to the mitochondrion and subsequently to the nucleus in response to oxidative stress and exerts an increased cytoprotective effect against oxidative damage. Detected in tau inclusions in brains from neurodegenerative disease patients.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Description
This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.
Applications for Park7/DJ-1 Antibody (4H4) [DyLight 488]
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
Optimal dilutions of this antibody should be experimentally determined.
Immunohistochemistry
Optimal dilutions of this antibody should be experimentally determined.
Knockout Validated
Optimal dilutions of this antibody should be experimentally determined.
Simple Western
Optimal dilutions of this antibody should be experimentally determined.
Western Blot
Optimal dilutions of this antibody should be experimentally determined.
Application Notes
This Park7(DJ-1), clone 4H4, antibody is useful for Immunocytochemistry/Immunofluorescence and Western blot, where a band can be seen at approximately 21 kDa.
The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein G purified
Formulation
50mM Sodium Borate
Preservative
0.05% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C in the dark.
Background: Park7/DJ-1
PARK7 is highly expressed in pancreas, kidney, skeletal muscle, liver, testis and heart, and is detected at slightly lower levels in placenta and brain. The protein is also detected in astrocytes, Sertoli cells, spermatogonia, spermatids and spermatozoa.
Defects in this gene cause the autosomal recessive neurodegenerative disorder early-onset Parkinson's disease 7.
Long Name
Parkinson Disease 7
Alternate Names
DJ-1, DJ1
Gene Symbol
PARK7
Additional Park7/DJ-1 Products
Product Documents for Park7/DJ-1 Antibody (4H4) [DyLight 488]
Product Specific Notices for Park7/DJ-1 Antibody (4H4) [DyLight 488]
DyLight (R) is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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