Patched 1/PTCH Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NB100-2451

Novus Biologicals, part of Bio-Techne
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NB100-2451
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Key Product Details

Species Reactivity

Validated:

Human, Mouse

Predicted:

Rat (100%). Backed by our 100% Guarantee.

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Peptide ELISA

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Concentration

0.5 mg/ml

View all Patched 1/PTCH Antibodies »

Product Specifications

Immunogen

This Patched 1/PTCH Antibody was developed against a peptide with sequence ELQDVECEERPR corresponding to C-Terminus according to NP_000255.1, NP_001077072.1, NP_001077071.1, NP_001077073.1, NP_001077074.1, NP_001077075.1, NP_001077076.1.

Specificity

This antibody is expected to recognise all reported isoforms. Variants (NP_001077073.1; NP_001077074.1; NP_001077075.1; NP_001077076.1) encode the same isoform.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Patched 1/PTCH Antibody

Immunocytochemistry/ Immunofluorescence: Patched 1/PTCH Antibody [NB100-2451]

Immunocytochemistry/ Immunofluorescence: Patched 1/PTCH Antibody [NB100-2451]

Immunocytochemistry/Immunofluorescence: Patched 1/PTCH Antibody [NB100-2451] - Immunofluorescence analysis of paraformaldehyde fixed NIH3T3 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL), showing Golgi staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL).
Immunohistochemistry-Paraffin: Patched 1/PTCH Antibody [NB100-2451]

Immunohistochemistry-Paraffin: Patched 1/PTCH Antibody [NB100-2451]

Immunohistochemistry-Paraffin: Patched 1/PTCH Antibody [NB100-2451] - Negative Control showing staining of paraffin embedded Human Cortex, with no primary antibody.
Immunocytochemistry/ Immunofluorescence: Patched 1/PTCH Antibody [NB100-2451]

Immunocytochemistry/ Immunofluorescence: Patched 1/PTCH Antibody [NB100-2451]

Immunocytochemistry/Immunofluorescence: Patched 1/PTCH Antibody [NB100-2451] - Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL), showing Golgi/cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL).
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Applications for Patched 1/PTCH Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

10 ug/mL

Immunohistochemistry

8 ug/mL

Immunohistochemistry-Paraffin

8 ug/mL

Peptide ELISA

Detection limit 1:16000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Patched 1/PTCH

Mutations of the human Patched tumor suppressor gene (PTCH) have been identified in individuals with the nevoid basal cell carcinoma syndrome (NBCCS) as well as in sporadic basal cell carcinomas and medulloblastomas.

Alternate Names

BCNS, HPE7, mes, NBCCS, ptc1, PTCH1

Entrez Gene IDs

5727 (Human)

Gene Symbol

PTCH1

UniProt

NP_000255.1 (Human)

Additional Patched 1/PTCH Products

Product Documents for Patched 1/PTCH Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Patched 1/PTCH Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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