Patched 1/PTCH Antibody - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-71662

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Human, Mouse, Rat

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

1.22 mg/ml

View all Patched 1/PTCH Antibodies »

Product Specifications

Immunogen

A genomic peptide made to a C-terminal region of the human Patched 1 protein (within residues 1200-1350). [Swiss-Prot Q13635]

Reactivity Notes

Rat reactivity reported in scientific literature (PMID: 30193838).

Localization

Membrane, Cytoplasm

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Patched 1/PTCH Antibody - BSA Free

Western Blot: Patched 1/PTCH AntibodyBSA Free [NBP1-71662]

Western Blot: Patched 1/PTCH AntibodyBSA Free [NBP1-71662]

Western Blot: Patched 1/PTCH Antibody [NBP1-71662] - Analysis of Patched 1 in human skeletal muscle protein.
Immunohistochemistry-Paraffin: Patched 1/PTCH Antibody - BSA Free [NBP1-71662]

Immunohistochemistry-Paraffin: Patched 1/PTCH Antibody - BSA Free [NBP1-71662]

Immunohistochemistry-Paraffin: Patched 1/PTCH Antibody [NBP1-71662] - IHC analysis of formalin fixed paraffin embedded tissue section of mouse lung using 1:50 dilution of PTCH antibody with HRP-DAB detection and hematoxylin counterstaining. The antibody generated a specific membrane-cytoplasmic staining in bronchiolar and alveolar epithelial cells with stronger signal towards the membranes of the cells.
Immunohistochemistry-Paraffin: Patched 1/PTCH Antibody - BSA Free [NBP1-71662]

Immunohistochemistry-Paraffin: Patched 1/PTCH Antibody - BSA Free [NBP1-71662]

Immunohistochemistry-Paraffin: Patched 1/PTCH Antibody [NBP1-71662] - IHC analysis of formalin fixed paraffin embedded tissue section of mouse lung using 1:50 dilution of PTCH antibody with HRP-DAB detection and hematoxylin counterstaining. The antibody generated a specific membrane-cytoplasmic staining in bronchiolar and alveolar epithelial cells with stronger signal towards the membranes of the cells.

Applications for Patched 1/PTCH Antibody - BSA Free

Application
Recommended Usage

Immunohistochemistry

1:50 - 1:250

Immunohistochemistry-Paraffin

1:50 - 1:250

Western Blot

1:2500
Application Notes
This Patched 1 antibody is useful in Western blot, where a band is seen ~161 kDa.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS

Format

BSA Free

Preservative

0.05% Sodium Azide

Concentration

1.22 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Patched 1/PTCH

Patched 1 (PTCH1) acts as a receptor for SHH, IHH (indian hedgehog) and DHH (desert hedgehog) in SHH/sonic hedgehog signaling pathway which is critical in embryonic development, tissue patterning, and cell-fate decisions. SHH pathway activation is triggered by binding of SHH to PTCH1 (glycosylation being necessary for binding), which in the absence of SHH suppresses the activity of SMO (Smoothened protein), and this SHH- PTCH1 binding or mutational inactivation of PTCH1 relieves the inhibition of SMO culminating in the activation of one or more of GLI1 transcription factors that regulate the expression of downstream targets. PTCH1 mutations or its aberrant expression may leads to developmental defects and a cancer-prone phenotype. Mice partially deficient in functional PTCH1 exhibit developmental abnormalities that are comparable to those of nevoid basal cell carcinoma syndrome patients and additionally, these mice develop tumors such as medulloblastomas, rhabdomyosarcomas, and basal cell carcinomas following UV or ionizing radiation exposure. PTCH1 germline mutations in humans are responsible for nevoid basal cell carcinoma syndrome, also called Gorlin syndrome characterized by various developmental malformations and a high predisposition to skin basal cell carcinoma. Besides basal cell nevus syndrome (BCNS), defects in PTCH1 have been linked to sporadic basal cell carcinoma (BCC) and holoprosencephaly type 7 (HPE7).

Alternate Names

BCNS, HPE7, mes, NBCCS, ptc1, PTCH1

Entrez Gene IDs

5727 (Human)

Gene Symbol

PTCH1

UniProt

Q13635 (Human)

Additional Patched 1/PTCH Products

Product Documents for Patched 1/PTCH Antibody - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Patched 1/PTCH Antibody - BSA Free

Manufactured by Genomic Antibody Technology™. GAT FAQs

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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